Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA-seq of metabolically labelled RNA by 4-thiouracil from mouse tissues

ABSTRACT: RNA-seq of metabolically labelled RNA by 4-thiouracil from mouse tissues

PROVIDER: PRJEB24362 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	ERR2241550.fastq.gz	Fastqsanger.gz
	ERR2241560.fastq.gz	Fastqsanger.gz
	ERR2241551.fastq.gz	Fastqsanger.gz
	ERR2241552.fastq.gz	Fastqsanger.gz
	ERR2241543.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 18

Similar Datasets

RNA-seq of metabolically labelled RNA by 4-thiouracil from mouse tissues

Project description:To identify cell type-specific RNA without cell sorting, mice expressing UPRT in a specific cell type were exposed to 4-thiouracil to label RNA only in the UPRT-expressing cells. Total RNA was extracted from tissues and treated with iodoacetamide followed by RNA-seq library preparation. The reads generated from 4-thiouracil-containing transcripts should contain T to C mismatches as a result of 4-thiouracil incorporations and thus can be identified as the transcripts generated in the UPRT-expressing cells.

2018-07-09 | E-MTAB-6353 | biostudies-arrayexpress

Metabolic labeling of Hek293 cells using 4-thiouracil

Project description:Hek293 cells were metabolically labelled using 4-thiouracil as described in (Schwalb et al, Science. 2016 Jun 3;352(6290):1225-8) but without fragmentation, and then bulk RNA was prepared for sequencing using the STRT method (Islam et al, Genome Res. 2011 Jul;21(7):1160-7). Samples were incubated in duplicate for 5, 15 and 30 minutes and included an unlabeled control representing the steady-state expression state.

2018-08-09 | GSE115813 | GEO

RNA-seq of biotin-labelled-miR190b pulldown

Project description:RNA-seq of biotin-labelled-miR190b pulldown

| PRJNA816541 | ENA

Data resource for fully 13C labelled and non-labelled plant tissues (part-I)

Project description:LC-MS/MS data files of fully 13C labelled and 12C plant tissues, which were utilized to determine the carbon element number for metabolite characterizations.

2018-11-08 | ST001095 | MetabolomicsWorkbench

Identification of metabolically distinct adipocyte progenitor cells in human adipose tissues

Project description:Identification of metabolically distinct adipocyte progenitor cells in human adipose tissues

| PRJNA529861 | ENA

Thiouracil Pulse-chase, Tachyzoites and Bradyzoites mRNA stability data

Project description:Pulse chase measurements using thiouracil (DTU) labeling via UPRT and chasing with uracil Data from tachyzoites is labeled "DTU Pulse Chase". Two independent pulse chase experiments were performed in tachyzoites, pulse chase 1 and 2. Duplicate arrays at each timepoint were performed for pulse chase 2 (2 a and b). Data from bradyzoites are labeled "DTU Bradyzoite Pulse Chase". Two independent pulse chase experiments were performed in bradyzoites and a single set of arrays were performed for each experiment. Just one chase timepoint was used in the bradyzoite experiments, the 2 hour chase. An RNA stablity experiment design type examines stability and/or decay of RNA transcripts. Keywords: RNA_stability_design

2005-07-16 | GSE2947 | GEO

TU-tagging: cell type specific RNA isolation from intact complex tissues

Project description:We show that the combination of spatially restricted uracil phosphoribosyltransferase (UPRT) expression with 4-thiouracil (4TU) delivery can be used to label and purify cell type specific RNA from intact complex tissues. This method is useful for isolating RNA from cell types that are difficult to isolate by dissection or dissociation methods and should work in any organism where UPRT can be spatially expressed, including mammals and other vertebrates.

2009-04-24 | GSE15445 | GEO

Study on the expression of long-chain non coding RNA and coding gene in patients with colorectal cancer

Project description:Interventions: lesion tissues vs. adjacent tissues of colorectal cancer patients:nil Primary outcome(s): RNA Study Design: Factorial

| 2693518 | ecrin-mdr-crc

mRNA stability as measured by thiouracil incorporation in the presence and absence of translational inhibitors

Project description:We measure the stability of mRNAs in rapidly dividing yeast by metabolic labeling with thiouracil and determine the effects on mRNA stability in the presence of various inhibitors of translation elongation and initiation.

2018-09-07 | GSE119560 | GEO

A MAFG-lncRNA axis links systemic nutrient abundance to hepatic glucose metabolism: RNA profiles of metabolically active tissues.

Project description:A MAFG-lncRNA axis links systemic nutrient abundance to hepatic glucose metabolism: RNA profiles of metabolically active tissues.

| PRJNA497100 | ENA

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data