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ABSTRACT: EMG produced TPA metagenomics assembly of the Resistome of effluents of wastewater treatment plants () data set.

PROVIDER: PRJEB24372 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6158320.fasta.gz	Fasta.gz
	SRR6158309.fasta.gz	Fasta.gz
	SRR6158308.fasta.gz	Fasta.gz
	SRR6158314.fasta.gz	Fasta.gz
	SRR6158313.fasta.gz	Fasta.gz

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Project description:Resistome of effluents of wastewater treatment plants

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Project description:The increase in human population and urbanization are resulting in an increase in the volume of wastewater and urban runoff effluents entering natural ecosystems. These effluents may contain multiple pollutants to which the biological response of aquatic organisms is still poorly understood mainly due to mixture toxicity and interactions with other environmental factors. In this context, RNA sequencing was used to assess the impact of a chronic exposure to wastewater treatment plant and stormwater effluents at the whole-transcriptome level and evaluate the potential physiological outcomes in the Asian clam Corbicula fluminea. We de-novo assembled a transcriptome from C. fluminea digestive gland and identified a set of 3,181 transcripts with altered abundance in response to water quality. The largest differences in transcriptomic profiles were observed between C. fluminea from the reference site and those exposed to wastewater treatment plant effluents. On both anthropogenically impacted sites, most differentially expressed transcripts were involved in signaling pathways in relation to energy metabolism such as mTOR and FoxO, suggesting an energy/nutrient deficit and hypoxic conditions. These conditions were likely responsible for damages to proteins and transcripts in response to wastewater treatment effluents whereas exposure to urban runoff might result in immune and endocrine disruptions. In absence of comprehensive chemical characterization, the RNAseq approach could provide information regarding the mode of action of pollutants and then be useful for the identification of which parameters must be studied at higher integration level in order to diagnose sites where the presence of complex and variable mixtures of chemicals is suspected.

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