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ACE biodegradation

ABSTRACT: Emerging biodegradation of the previously persistent artificial sweetener acesulfame in biological wastewater treatment

PROVIDER: PRJEB24423 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR2247480.fastq.gz	Fastqsanger.gz
	ERR2247481.fastq.gz	Fastqsanger.gz
	ERR2247482.fastq.gz	Fastqsanger.gz
	ERR2247702_1.fastq.gz	Fastqsanger.gz
	ERR2247702_2.fastq.gz	Fastqsanger.gz

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Alteration of global transcription by the artificial sweetener acesulfame K in Acinetobacter baumannii AB5075

Project description:We perform RNA-seq comparing a treatment with 1.33% acesulfame K to a mock treatment in A. baumannii AB5075 in order to describe the alterations in global transcription produced by this artificial sweetener. A. baumannii AB5075 cultures were grown in LB medium in the presence of 1.33% acesulfame K or a water control (3 biological replicates per condition) until reaching mid-exponential phase. At this point, cells were harvested, treated with RNAlate for preservation of total RNA and stored at -80 C. After that, total RNA was extracted from each sample. As a result, 212 genes appeared upregulated in the presence of acesulfame K, whereas 252 were downregulated. In highlights, the gene functional groups showing the strongest transcriptional alterations were those related to twtching motility and natural transformation (downregulation of genes involves in biogenesis and control of Type IV pili and com genes), as well as genes involved in iron acquisition and siderophore biosynthesis and an uncharacterised gene cluster that might be involved in detoxification of sulfonamide compounds.

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