Project description:Carboxy-terminally tagged MOZ (Flag-V5-BIO tagged) was detected by ChIP-seq using anti-V5 antibody (Sigma, A7345) to precipitate chromatin associated with MOZ
Project description:In this experiment, we performed anti-Flag, anti-H3K4me3 and anti-H3K27ac Cut&Run on DU145 cells with endogenous BRD4 knockout and re-introduced of Flag-tagged WT- or AA-BRD4.
Project description:Isolation of IMP1 bound mRNAs. Flag-tagged IMP1 was expressed in HEK293 cells. Flag tagged IMP1 was immunoprecipitated and mRNAs isolated. As controls HEK293 cells that do not express Flag-tagged IMP1 was included.
