Project description:In the present study we aimed to develop a custom made cDNA microarray, the CodStress array, for Atlantic cod to be used as a diagnostic tool in environmental monitoring of polluted waters. We wanted to investigate if the gene expression profiles would reflect environmental levels and composition of pollutants in two saltwater recipients, S?rfjorden and Store Lungeg?rdsvann.
Project description:Monitoring microbial communities can aid in understanding the state of these habitats. Environmental DNA (eDNA) techniques provide efficient and comprehensive monitoring by capturing broader diversity. Besides structural profiling, eDNA methods allow the study of functional profiles, encompassing the genes within the microbial community. In this study, three methodologies were compared for functional profiling of microbial communities in estuarine and coastal sites in the Bay of Biscay. The methodologies included inference from 16S metabarcoding data using Tax4Fun, GeoChip microarrays, and shotgun metagenomics.
