Project description:Annotation of small RNAs from 11 Drosophila species for the purpose of non-coding RNA annotation and comparative genomics assessment.
Project description:Paper title: Insights into the antimicrobial activities and metabolomes of Aquimarina (Flavobacteriaceae, Bacteroidetes) species from the rare marine biosphere
Author List: Silva, SG, Paula, P, da Silva, J.P, Mil-Homens, D, Teixeira, MC, Fialho, A, Costa, R, Keller-Costa, T
PubMedID:
Brief description of the data submitted: RAW Files and .mzXML Files used to generate Figures 4 and 5.
Sample Collection Method: Supernatants of 120 mL of Aquimarina spp. cultures grown in 1:2 diluted Marine Broth at 24 degrees Celsius in an incubator (Labtron) with 120 rpm orbital shaking for two days.
Sample Extraction Method: Liquid extracts; Solid phase extraction (HLB cartridges); Elution with methanol (100%); Eluates were evaporated in a gentle flux of nitrogen gas and reconstituted in 500 microlitres of methanol-water (50:50 v/v).
Ionization Souce and Polarity: Heated Electro-Spray Ionization source (HESI-II; Thermo Scientific). Positive Ionization Polarity.
Chromatography and Phase: Thermo Scientific Accucore RP-18 column (2.1 times 100 mm, 2.6 microm), Water-Acetonitrile gradient with 0.1% formic acid.
Project description:Curration of small RNAs from four melanogaster-subgroup species (Drosophila simulans, Drosophila sechellia, Drosophila erecta, and Drosophila yakuba) for the purpose of non-coding RNA annotation and comparative genomics assessment.
