Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNAi of signal transduction components in HCT116 cells

ABSTRACT: RNAi of signal transduction components in HCT116 cells

PROVIDER: PRJEB2536 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other
	ERR034229.fastq.gz	Fastqsanger.gz
	ERR034230.fastq.gz	Fastqsanger.gz
	ERR034231.fastq.gz	Fastqsanger.gz
	ERR034232.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 14

Similar Datasets

RNAi of signal transduction components in HCT116 cells

Project description:We performed RNAi knockdown experiments on 7 different signal transduction components of the Wnt pathway in HCT116 colon cancer cells and prepared RNA libraries from the poly-adenylated fraction of the RNA. SOLiD sequencing of strand-specific, barcoded transcriptome libraries yielded expression profiles allowing us to extract common pathway signatures and enabeling us to perform susequent nested effects modelling. the resulting pathway models yields new insight in the pathway topology in colon cancer.

2014-04-18 | E-MTAB-651 | biostudies-arrayexpress

RNAi of signal transduction components in Drosophila S2 cells

Project description:RNAi of signal transduction components in Drosophila S2 cells

| PRJEB2222 | ENA

RNA-seq of Drosophila S2 cells after RNAi knock-down of signal transduction components

Project description:We performed RNAi knockdown experiments on 15 different signal transduction components in Drosophila S2 cells and prepared RNA libraries from the poly-adenylated fraction of the RNA. SOLiD sequencing of strand-specific, barcoded transcriptome libraries yielded expression profiles allowing us to extract common pathway signatures and indicated that Cka may function as novel regulator in Ras/MAPK signaling

2011-07-08 | E-MTAB-315 | biostudies-arrayexpress

[E-MTAB-315] RNA-seq of Drosophila S2 cells after RNAi knock-down of signal transduction components

Project description:RNAi of signal transduction components in Drosophila S2 cells We performed RNAi knockdown experiments on 15 different signal transduction components in Drosophila S2 cells and prepared RNA libraries from the poly-adenylated fraction of the RNA. SOLiD sequencing of strand-specific, barcoded transcriptome libraries yielded expression profiles allowing us to extract common pathway signatures and indicated that Cka may function as novel regulator in Ras/MAPK signaling. ArrayExpress Release Date: 2011-07-08 Person Roles: investigator Person Last Name: Boutros Person First Name: Michael Person Email: m.boutros@dkfz.de Person Address: Deutsches Krebsforschungszentrum, Signaling and Functional Genomics (B110), Im Neuenheimer Feld 580, 69120 Heidelberg, Germany Person Affiliation: Signaling and Functional Genomics, DKFZ Person Roles: investigator Person Last Name: Sandmann Person First Name: Thomas Person Email: t.sandmann@dkfz.de Person Address: Deutsches Krebsforschungszentrum, Signaling and Functional Genomics (B110), Im Neuenheimer Feld 580, 69120 Heidelberg, Germany Person Affiliation: Signaling and Functional Genomics, DKFZ Person Roles: investigator Person Last Name: Horn Person First Name: Thomas Person Email: t.horn@dkfz.de Person Address: Deutsches Krebsforschungszentrum, Signaling and Functional Genomics (B110), Im Neuenheimer Feld 580, 69120 Heidelberg, Germany Person Affiliation: Signaling and Functional Genomics, DKFZ Person Roles: submitter Person Last Name: Kerr Person First Name: Grainne Person Email: g.kerr@dkfz.de Person Address: Deutsches Krebsforschungszentrum, Signaling and Functional Genomics (B110), Im Neuenheimer Feld 580, 69120 Heidelberg, Germany Person Affiliation: Signaling and Functional Genomics, DKFZ

2011-07-14 | GSE30602 | GEO

Subcellular location defines GPCR signal transduction

Project description:Subcellular location defines GPCR signal transduction

| PRJNA915973 | ENA

Genome wide extracellular signal-regulated kinase (ERK) pathway components interaction with human genome

2016-08-18 | GSE65323 | GEO

Expression Profile of Signal Transduction Components in a Sugarcane Population Segregated for Sugar Content

Project description:Sucrose is the major product of photosynthesis in many higher plants. It is transported from the source tissue through the phloem to various sink tissues to support plant growth, development and reproduction. Knowledge of the signal transduction pathways involved in carbohydrate metabolism is limited. Genes involved in the regulation of sucrose synthesis and accumulation could be used as tools in the development of genetically manipulated plants with higher sucrose content or aid in breeding programs as molecular markers. Using the SUCEST (Sugarcane EST) database information and a microarray approach, the expression profiles of 1920 sugarcane (Saccharum spp.) genes encoding signal transduction elements, transcription factors and stress-related proteins were analyzed. In order to assess their involvement in sucrose metabolism, the expression profiles of individuals from a sugarcane population segregated for sugar content were compared. From a population of 500 F1 individuals, the seven with the highest and the seven with the lowest sugar contents were selected, pooled and profiled using cDNA microarrays. Twenty-four genes were identified as differentially expressed. Five of them were more expressed in the plants with the highest sugar contents and 19 in the plants with the lowest sugar contents. RNA-blot hybridization was used to validate the expression profiles obtained for individuals in each group. These genes were shown to have differential expression throughout the growing season and in the different tissues. Our data supports the view that sugar levels modulate a complex signal transduction network that seems to involve responses related to stress. Keywords: Segregated population

2006-02-14 | GSE4233 | GEO

Genome wide extracellular signal-regulated kinase (ERK) pathway components interaction with human genome

Project description:Several recent studies, including ours, show that most components of signal transduction cascades including the extracellular signal-regulated protein kinase (ERK), that once were thought to act predominantly in cytoplasm, are in fact recruited to chromatin and are integral components of transcriptional complexes. However, their distribution along whole genome remains uncovered. Here we investigate genome wide recruitment of the ERK pathway components using chromatin immunoprecipitations (ChIP) followed by deep sequencing, ChIP-Seq. Hela-S3 cells were starved for 48 hours (control) and stimulated with EGF at concentration of 100ng/mL for 20 minutes. Cells were fixed with formaldehyde, chromatin isolated and subjected to ChIP reaction. Two technical replicates of ChIP reaction followed by sequencing were performed.

2015-08-01 | E-GEOD-65323 | biostudies-arrayexpress

RNAi components regulate the micoparasitism in Trichoderma atroviride

Project description:RNAi components regulate the micoparasitism in Trichoderma atroviride

| PRJNA785614 | ENA

RNAi components regulate the microparasitism in Trichoderma atroviride

Project description:RNAi components regulate the microparasitism in Trichoderma atroviride

| PRJNA810376 | ENA