Project description:Determination of the complete genome of European mountain ash ringspot-associated emaravirus from Sorbus intermedia reveals two additional genome segments
Project description:ASH-1 orthologs are H3K36-specific methyltransferases that are conserved from fungi to humans but are poorly understood, in part because they are typically essential for viability. Here we examine the H3K36 methylation pathway of Neurospora crassa, which we find has just two H3K36 methyltransferases, ASH-1 and RNA polymerase II-associated SET-2. Our investigation of the interplay between SET-2 and ASH-1 uncovered a regulatory mechanism connecting ASH-1-catalyzed H3K36 methylation to repression of poorly transcribed genes. Our findings provide new insight into ASH-1 function, H3K27me2/3 establishment, and repression at facultative heterochromatin.
Project description:We used RNA-seq in a derived European Drosophila melanogaster population from Germany (MU) to examine coding gene expression variation in the larval fat body during the late wandering third instar stage.
