Project description:Palatine tonsils are secondary lymphoid organs that are strategically positioned in the oropharynx to secure a first line of defense against oral pathogens. Specialized immune-interacting fibroblasts, generally termed fibroblastic reticular cells (FRC), underpin distinct microenvironments within lymphoid organs to compartmentalize and direct the efficient interaction and activation of immune cells. As a particular anatomical property, palatine tonsils harbor a reticular-shaped lymphoepithelium that generates an antigen sampling zone in the crypts. While the histological ultrastructure and the immune cell composition of human palatine tonsils has been elaborated in detail, the molecular identity of the diverse stromal cell compartments including FRC and the specialized lymphoepithelium remains largely unknown. Here, we have employed single cell transcriptomics and extensive flow cytometric analyses to unveil the molecular identity of tonsillar cells and to disentangle the heterogeneity of fibroblast and epithelial cell subsets in palatine tonsils. Our results reveal a remarkable conservation of stromal cell organization and molecularly-defined subsets in infant and adult human palatine tonsils.
Project description:The use of single-cell sequencing technology for single-cell transcriptomics studies in pigs is expanding progressively. However, the comprehensive classification of cell types across different anatomical tissues and organs of pigs in multiple datasets remains relatively limited. This study centers on an investigation using single-cell sequencing technology in Bama pigs, where unique marker genes and their corresponding transcriptomic profiles have been identified for diverse cell types across distinct anatomical tissues and organs, encompassing subcutaneous fat, visceral fat, skeletal muscle, liver, spleen, lung, and kidney. Using comprehensive data analyses, we discovered the widespread cellular diversity inherent in different anatomical tissues and organs of Bama pigs. This study not only provides a pivotal dataset for physiological research on Bama pigs but also contributes to the verification and prediction of key marker genes in the single-cell transcriptomics of these tissues and organs. Furthermore, the data and methodologies that were used in this study underscore our commitment to enhancing the authenticity of cell type identification and ensuring the consistency in cell type allocation.
2023-12-22 | GSE241555 | GEO
Project description:Transcriptome data of Dangshen different organs
Project description:Sexual Schmidtea mediterranea Trunk Regeneration. Sexual biotype Schmidtea mediterranea trunk fragments were sequenced at 0 day, 3 day, 5 day and 7 day post amputation along with whole worm juvenile animals in order identify sexual specific transcripts. Juvenile worms lack sex organs, testes and ovaries present in D0 trunk fragments. Days 3-7 represent time points of degeneration and regeneration of the sexual organs, testes and ovaries.
Project description:To correlate transcriptomics profile with metabolomics profile, gene expression of root and aerial organs from four phenotypically (metabolomics) diverse ecotypes were compared.