Project description:Draft Genome Sequence of Rhizobium sp. strain T2.26MG-112.2 isolated from 492.6 meter deep on the subsurface of the Iberian Pyrite Belt.
Project description:Draft Genome Sequence of Brevundimonas sp. strain T2.26MG-97 Isolated from X Meter Deep on the Subsurface of the Iberian Pyrite Belt
Project description:Publication of 4 different bacterial strains from the genera Cellullomonas, Propionicimonas, Rhodoplanes and Tessaracoccus isolated from the deep subsurface of the Iberian Pyrite Belt (IPB)
Project description:Coevolutionary change requires reciprocal selection between interacting species, i.e., that the partner genotypes that are favored in one species depend on the genetic composition of the interacting species. Coevolutionary genetic variation is manifested as genotype ´ genotype (G ´ G) interactions for fitness from interspecific interactions. Although quantitative genetic approaches have revealed abundant evidence for G ´ G interactions in symbioses, the molecular basis of this variation remains unclear. Here we study the molecular basis of G ´ G interactions in a model legume-rhizobium mutualism using gene expression microarrays. We find that, like quantitative traits such as fitness, variation in the symbiotic transcriptome may be partitioned into additive and interactive genetic components. Our results suggest that plant genetic variation is the largest influence on nodule gene expression, and that plant genotype and the plant genotype ´ rhizobium genotype interaction determine global shifts in rhizobium gene expression that in turn feedback to influence plant fitness benefits. Moreover, the transcriptomic variation we uncover implicates regulatory changes in both species as drivers of symbiotic gene expression variation. Our study is the first to partition genetic variation in a symbiotic transcriptome, and illuminates potential molecular routes of coevolutionary change. We assayed gene expression using three biological replicates for each plant genotype × rhizobium genotype combination (4 combinations) for a total of 12 chips.
Project description:To examine how the Arabidopsis root development responds to the Rhizobium sp. IRBG74 treatment at the molecular level, we performed RNA-seq experiments. Our RNA-seq results suggest that expression of genes mainly involved in auxin signaling, cell wall and cell membrane integrity and transport is altered in response to colonization by Rhizobium sp. IRBG74.
Project description:Genomic information of Rhodoplanes piscinae sp. strain T2.26MG-98 like microorganisms that have been isolated from the deep-subsurface of the Iberian Pyrite Belt (Southwester Spain) in strict anaerobic conditions.
