Project description:To explore brain neuropeptidic functions in behavioral regulation, a label-free quantitative strategy was employed to compare neuropeptidomic variations between behavioral phenotypes (nurse bees, nectar foragers, and pollen foragers) and the two honeybee species (Apis mellifera ligustica and Apis cerana cerana).
Project description:he brain is a vital organ in regulating complex social behaviors of honeybees including learning and memory. Knowledge of how brain membrane proteins and their phosphorylation underlie the age-related behavioral polyethism is still lacking. We presented the first comprehensive profiling and comparison of brain membrane proteome and phosphoproteome across different ages of adult worker bees in two strains of honeybee (Apis mellifera ligustica): Italian bee (ITB) and royal jelly bee (RJB), a line selected for increased RJ outputs over 4 decades.
Project description:In Apis mellifera, the female eggs can develop into workers or queen depending on the diet offered during early development. The outputs of the developed honeybee females are two morphs with particular morphological traits and related physiology. The differential feeding regime experienced by the queen and the worker larvae of the honeybee Apis mellifera shapes a complex endocrine response cascade that ultimately sets up differences in brain morphologies. Herein we report on aspects of brain morphogenesis during larval development and the brain gene expression signature of fourth instar larvae (L4) of both castes, a developmental stage characterized by the greatest differences in juvenile hormone (JH) titers between castes Using results from the hybridization of whole genome-based oligonucleotide arrays with RNA samples from brain of fourth instar larvae honeybees of both castes we present a list of differentially expressed genes.
Project description:Female larvae of the honeybee (Apis mellifera) develop into either queens or workers depending on nutrition during larval development. This nutritional stimulus triggers different developmental trajectories, resulting in adults that differ in physiology, behaviour and life-span. To understand how these developmental trajectories are established we have undertaken a comprehensive analysis of differential gene expression throughout larval development.
Project description:Female larvae of the honeybee (Apis mellifera) develop into either queens or workers depending on nutrition during larval development. This nutritional stimulus triggers different developmental trajectories, resulting in adults that differ in physiology, behaviour and life-span. To understand how these developmental trajectories are established we have undertaken a comprehensive analysis of differential gene expression throughout larval development.
Project description:In Apis mellifera, the female eggs can develop into workers or queen depending on the diet offered during early development. The outputs of the developed honeybee females are two morphs with particular morphological traits and related physiology. The differential feeding regime experienced by the queen and the worker larvae of the honeybee Apis mellifera shapes a complex endocrine response cascade that ultimately sets up differences in brain morphologies. Herein we report on aspects of brain morphogenesis during larval development and the brain gene expression signature of fourth instar larvae (L4) of both castes, a developmental stage characterized by the greatest differences in juvenile hormone (JH) titers between castes Using results from the hybridization of whole genome-based oligonucleotide arrays with RNA samples from brain of fourth instar larvae honeybees of both castes we present a list of differentially expressed genes. Analysis used one dye-swap combination to compare workers and queens brain development at fourth instar larvae when juvenile hormone titers is higher in queens.
Project description:Female larvae of the honeybee (Apis mellifera) develop into either queens or workers depending on nutrition during larval development. This nutritional stimulus triggers different developmental trajectories, resulting in adults that differ in physiology, behaviour and life-span. To understand how these developmental trajectories are established we have undertaken a comprehensive analysis of differential gene expression throughout larval development. Gene expression of honeybee queen and worker larval samples was analysed at 60 hours with high-throughout sequencing
Project description:Female larvae of the honeybee (Apis mellifera) develop into either queens or workers depending on nutrition during larval development. This nutritional stimulus triggers different developmental trajectories, resulting in adults that differ in physiology, behaviour and life-span. To understand how these developmental trajectories are established we have undertaken a comprehensive analysis of differential gene expression throughout larval development. Gene expression of honeybee queen and worker larval samples was analysed at seven time points during larval development (6 hr, 12 hr, 36 hr, 60 hr, 84 hr, 108 hr and 132 hr)
Project description:We have identified a honeybee (Apis mellifera) odorant receptor (Or) for the queen substance 9-oxo-2-decenoic acid (9-ODA) from four candidate sex pheromone odorant receptors from the honeybee genome based on their biased expression in drone antennae. Keywords: Tissue Comparison