Project description:We isolated a thermophilic hydrogenotrophic methanogen, Methanothermobacter sp. strain CaT2, which is able to aggregate and utilize formate. Here, we report the complete genome sequence of this organism.

| S-EPMC3757452 | biostudies-literature

Complete Genomic Sequence of the Thermophilic and Hydrogenotrophic Methanogen Methanothermobacter sp. Strain KEPCO-1.

Project description:Here, we describe the complete genome of Methanothermobacter sp. strain KEPCO-1, a thermophilic and hydrogenotrophic methanogen that was isolated from an anaerobic digester in Seoul, Republic of Korea. The genome of KEPCO-1 shares 96.98% of its sequence with Methanothermobacter marburgensis strain DSM 2133 and consists of 1,741,029 bp, with 1,822 protein-coding genes, 44 noncoding RNAs, and a GC content of 48.47%. The development of this genome will facilitate future genomic studies of KEPCO-1.

| S-EPMC6940278 | biostudies-literature

Complete Genomic Sequence of the Thermophilic Hydrogen-Oxidizing Methanogen Methanothermobacter tenebrarum Strain RMAS<sup>T</sup>.

Project description:Methanothermobacter tenebrarum strain RMAST has a complete genomic length of 1,472,762 bp, a GC content of 42.1%, 1,599 coding DNA sequences (CDSs), 1 CRISPR array, 3 rRNAs, and 38 tRNAs.

| S-EPMC9302168 | biostudies-literature

Effect of biogas slurry combined with biochar on soil microbial function gene

Project description:The functional diversity of soil microbial communities was explored for a poplar plantation, which was treated solely with biogas slurry, or combined with biochar at different fertilization intensities over several years.

2020-03-30 | GSE133800 | GEO

Genome Analyses and Genome-Centered Metatranscriptomics of Methanothermobacter wolfeii Strain SIV6, Isolated from a Thermophilic Production-Scale Biogas Fermenter.

Project description:In the thermophilic biogas-producing microbial community, the genus Methanothermobacter was previously described to be frequently abundant. The aim of this study was to establish and analyze the genome sequence of the archaeal strain Methanothermobacter wolfeii SIV6 originating from a thermophilic industrial-scale biogas fermenter and compare it to related reference genomes. The circular chromosome has a size of 1,686,891 bases, featuring a GC content of 48.89%. Comparative analyses considering three completely sequenced Methanothermobacter strains revealed a core genome of 1494 coding sequences and 16 strain specific genes for M. wolfeii SIV6, which include glycosyltransferases and CRISPR/cas associated genes. Moreover, M. wolfeii SIV6 harbors all genes for the hydrogenotrophic methanogenesis pathway and genome-centered metatranscriptomics indicates the high metabolic activity of this strain, with 25.18% of all transcripts per million (TPM) belong to the hydrogenotrophic methanogenesis pathway and 18.02% of these TPM exclusively belonging to the mcr operon. This operon encodes the different subunits of the enzyme methyl-coenzyme M reductase (EC: 2.8.4.1), which catalyzes the final and rate-limiting step during methanogenesis. Finally, fragment recruitment of metagenomic reads from the thermophilic biogas fermenter on the SIV6 genome showed that the strain is abundant (1.2%) within the indigenous microbial community. Detailed analysis of the archaeal isolate M. wolfeii SIV6 indicates its role and function within the microbial community of the thermophilic biogas fermenter, towards a better understanding of the biogas production process and a microbial-based management of this complex process.

| S-EPMC7022856 | biostudies-literature

Draft Genome Sequence of Methanothermobacter thermautotrophicus WHS, a Thermophilic Hydrogenotrophic Methanogen from Washburn Hot Springs in Yellowstone National Park, USA.

Project description:A thermophilic methanogen was enriched in coculture from Washburn Hot Springs (Yellowstone National Park, USA), grown on carbon dioxide and hydrogen, and subsequently sequenced. The reconstructed 1.65-Mb genome sequence for Methanothermobacter thermautotrophicus WHS contributes to our understanding of hydrogenotrophic, CO2-reducing methanogenesis in geothermal ecosystems.

| S-EPMC7862951 | biostudies-literature

Hydrogenotrophs-Based Biological Biogas Upgrading Technologies.

Project description:Biogas produced from anaerobic digestion consists of 55-65% methane and 35-45% carbon dioxide, with an additional 1-2% of other impurities. To utilize biogas as renewable energy, a process called biogas upgrading is required. Biogas upgrading is the separation of methane from carbon dioxide and other impurities, and is performed to increase CH4 content to more than 95%, allowing heat to be secured at the natural gas level. The profitability of existing biogas technologies strongly depends on operation and maintenance costs. Conventional biogas upgrading technologies have many issues, such as unstable high-purity methane generation and high energy consumption. However, hydrogenotrophs-based biological biogas upgrading offers an advantage of converting CO2 in biogas directly into CH4 without additional processes. Thus, biological upgrading through applying hydrogenotrophic methanogens for the biological conversion of CO2 and H2 to CH4 receives growing attention due to its simplicity and high technological potential. This review analyzes the recent advance of hydrogenotrophs-based biomethanation processes, addressing their potential impact on public acceptance of biogas plants for the promotion of biogas production.

| S-EPMC9085624 | biostudies-literature

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