Project description:Genetic studies have shown essential functions of N-glycosylation during infection of the plant pathogenic fungi, however, systematic roles of N-glycosylation in fungi is still largely unknown. Biological analysis demonstrated N-glycosylated proteins were widely present at different development stages of Magnaporthe oryzae and especially strong in the appressorium and invasive hypha.A large-scale quantitative proteomics analysis was then performed to explore the roles of N-glycosylation in M. oryzae.
Project description:Magnaporthe oryzae is the causative agent of the rice blast, the most relevant rice disease worldwide. To date expression analysis on rice infected with Magnaporthe oryzae have been carried out only with the strains FR13 (leaf) and Guy 11 (root). However different strains of Magnaporthe are present in the environment leading to different rice responses at molecular level. To gain more insight on the unknown molecular mechanisms activated by different Magnaporthe strains during rice defense, a global expression analysis was performed by using the GeneChip® Rice Genome Array. To identify rice genes differentially regulated upon infection by Magnaporthe isolates, inoculation with different strains were performed and samples were collected 24 hours post infection.
Project description:The molecular mechanisms underlying infection of rice host system with compatible M. oryzae isolates at 12, 24, 48 and 72 hours post innoculation (hpi) are investigated in the study. In addition, the identification of highly commonly upregulated genes by both isolates of M. oryzae may be useful candidates for M.oryzae inducible promoter characterization We used microarrays to detail the global programme of gene expression underlying M. oryzae infection during compatible host-pathogen interaction
Project description:Magnaporthe oryzae is the causative agent of the rice blast, the most relevant rice disease worldwide. To date expression analysis on rice infected with Magnaporthe oryzae have been carried out only with the strains FR13 (leaf) and Guy 11 (root). However different strains of Magnaporthe are present in the environment leading to different rice responses at molecular level. To gain more insight on the unknown molecular mechanisms activated by different Magnaporthe strains during rice defense, a global expression analysis was performed by using the GeneChip® Rice Genome Array. To identify rice genes differentially regulated upon infection by Magnaporthe isolates, inoculation with different strains were performed and samples were collected 24 hours post infection. RNA were obtained from leaf samples after inoculation of rice 2 week-old plantlets with the following strains: rice isolates Magnaporthe oryzae FR13 and CL367, non-adapted strain BR32, isolated from wheat, and Magnaporthe grisea BR29 isolated from crabgrass. Treated and control (mock) rice leaves (cv. Nipponbare) were collected 24 hours post inoculation. Three biological replicates for each interaction type and the corresponding mock were extracted and analysed independently with the GeneChip® Rice Genome Array.
Project description:Concomitant sRNA and mRNAseq was carried out to elucidate the reprogramming occurring during Magnaporthe oryzae - Brachypodium distachyon interaction in three different setups: biotrophic stage of leaf infection (Leaf 2 DPI), necrotrophic stage of leaf infection (Leaf 4 DPI) and finally root infection (Root).
Project description:Concomitant sRNA and mRNAseq was carried out to elucidate the reprogramming occurring during Magnaporthe oryzae - Brachypodium distachyon interaction in three different setups: biotrophic stage of leaf infection (Leaf 2 DPI), necrotrophic stage of leaf infection (Leaf 4 DPI) and finally root infection (Root).
Project description:To investigate the role of iron excess in rice immune responses to Magnaporthe oryzae infection. Gene expression profiling analysis were performed using data obtained from RNA-seq of rice plants grown in differential iron supply and challenged with Magnaporthe oryzae spores.
Project description:We report that rice inoculated with Magnaporthe oryzae at different temperatures and analysis of the whole genome expression to shed light on temperature-mediated rice disease resistance.
Project description:This SuperSeries is composed of the following subset Series: GSE8517: Magnaporthe oryzae gene expression during biotrophic invasion of rice using version 2 of the Agilent Magnaporthe grisea Array (G4137B). GSE8518: Rice gene expression during biotrophic invasion by the rice blast fungus Magnaporthe oryzae using the Agilent Rice Array (G4138A). Keywords: SuperSeries Refer to individual Series