Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Molecular analysis of meso- and thermophilic microbiota associated with anaerobic biowaste degradation

ABSTRACT: Molecular analysis of meso- and thermophilic microbiota associated with anaerobic biowaste degradation

PROVIDER: PRJEB2731 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Molecular analysis of meso- and thermophilic microbiota associated with anaerobic biowaste degradation.

Project description:BackgroundMicrobial anaerobic digestion (AD) is used as a waste treatment process to degrade complex organic compounds into methane. The archaeal and bacterial taxa involved in AD are well known, whereas composition of the fungal community in the process has been less studied. The present study aimed to reveal the composition of archaeal, bacterial and fungal communities in response to increasing organic loading in mesophilic and thermophilic AD processes by applying 454 amplicon sequencing technology. Furthermore, a DNA microarray method was evaluated in order to develop a tool for monitoring the microbiological status of AD.ResultsThe 454 sequencing showed that the diversity and number of bacterial taxa decreased with increasing organic load, while archaeal i.e. methanogenic taxa remained more constant. The number and diversity of fungal taxa increased during the process and varied less in composition with process temperature than bacterial and archaeal taxa, even though the fungal diversity increased with temperature as well. Evaluation of the microarray using AD sample DNA showed correlation of signal intensities with sequence read numbers of corresponding target groups. The sensitivity of the test was found to be about 1%.ConclusionsThe fungal community survives in anoxic conditions and grows with increasing organic loading, suggesting that Fungi may contribute to the digestion by metabolising organic nutrients for bacterial and methanogenic groups. The microarray proof of principle tests suggest that the method has the potential for semiquantitative detection of target microbial groups given that comprehensive sequence data is available for probe design.

| S-EPMC3408363 | biostudies-literature

Thermophilic anaerobic digester

Project description:Uncovering core populations in thermophilic anaerobic digesters using metagenomic analysis

| PRJNA765437 | ENA

NaphS2 Gene Expression During Anaerobic Degradation of Naphthalene

2010-07-22 | GSE12323 | GEO

Anaerobic Degradation of Phenylacetate By the Hyperthermophilic Archaeon, Ferroglobus placidus

Project description:Comparison of acetate- to phenylacetate-grown F. placidus cells to identify genes that are potentially involved in anaerobic phenylacetate degradation by this unique hypertherophilic archaeon.

2014-10-01 | GSE59466 | GEO

NaphS2 Gene Expression During Anaerobic Degradation of Naphthalene

Project description:Degradation of polycyclic aromatic hydrocarbons (PAHs) such as naphthalene by anaerobic microorganisms is poorly understood. Strain NaphS2, an anaerobic sulfate reducing marine delta-proteobacterium is capable of using naphthalene and the aromatic compound benzoate, as well as pyruvate, as an electron donors in the presence of sulfate. In order to identify genes involved in the naphthalene degradation pathway, we compared gene expression in NaphS2 during growth on benzoate vs. pyruvate, naphthalene vs. pyruvate, and naphthalene vs benzoate. For each experimental set, aRNA from NaphS2 was labelled Cy5 (experiment) or Cy3(control) with three biological replicates hybridized in duplicate. In addition, because of the size of the predicted genome of NaphS2, ORFs were divided into two separate array designs, designated set1 and set2, such that set1 and set2 represent two separate array designs (probe sets) to be treated separately in statistical analysis.

2010-07-21 | E-GEOD-12323 | biostudies-arrayexpress

Mesophilic and thermophilic anaerobic digestion bioreactors

Project description:Mesophilic and thermophilic anaerobic digestion bioreactors Raw sequence reads

| PRJNA1185473 | ENA

JXX Anaerobic hrydrocarbon degradation

Project description:JXX Anaerobic hrydrocarbon degradation Raw sequence reads

| PRJNA839616 | ENA

Anaerobic degradation of BaP

Project description:Anaerobic degradation of BaP Genome sequencing and assembly

| PRJNA554458 | ENA

Polypropylene degradation digester

Project description:Potential for anaerobic degradation of polypropylene in thermophilic digester sludge

| PRJEB40381 | ENA

Anaerobic degradation of soil macrogenome

Project description:Anaerobic degradation of soil metagenome Raw sequence reads

| PRJNA880491 | ENA

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data