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Endoderm Differentiation - Single Cell RNA-sequencing

ABSTRACT: Endoderm Differentiation - Single Cell RNA-sequencing

PROVIDER: PRJEB27930 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR2713110_1.fastq.gz	Fastqsanger.gz
	ERR2713110_2.fastq.gz	Fastqsanger.gz
	ERR2713120_1.fastq.gz	Fastqsanger.gz
	ERR2713120_2.fastq.gz	Fastqsanger.gz
	ERR2713130_1.fastq.gz	Fastqsanger.gz

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Single-Cell RNA Sequencing Reveals Metallothionein Heterogeneity during hESC Differentiation to Definitive Endoderm [RNA-Seq]

Project description:Differentiation of human pluripotent stem cells toward definitive endoderm (DE) is the critical first step for generating cells comprising organs such as the gut, liver, pancreas and lung. This in-vitro differentiation process generates a heterogeneous population with a proportion of cells failing to differentiate properly and maintaining expression of pluripotency factors such as Oct4. RNA sequencing of single cells collected at four time points during a 4-day DE differentiation identified high expression of metallothionein genes in the residual Oct4-positive cells that failed to differentiate to DE. Using X-ray fluorescence microscopy and multi-isotope mass spectrometry, we discovered that high intracellular zinc level corresponds with persistent Oct4 expression and failure to differentiate. This study improves our understanding of the cellular heterogeneity during in-vitro directed differentiation and provides a valuable resource to improve DE differentiation efficiency.

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