Ontology highlight
ABSTRACT:
PROVIDER: PRJEB28591 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| chr10_eva.vcf.gz | Vcf | |||
| chr10_eva.vcf.gz.tbi | Vcf | |||
| chr1_eva.vcf.gz | Vcf | |||
| chr1_eva.vcf.gz.tbi | Vcf | |||
| chr2_eva.vcf.gz | Vcf |
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Project description:The hydroxycinnamic acid amides (HCAAs) are a diverse group of plant specialized phenylpropanoid metabolites distributed widely in the plant kingdom and are known to be involved in tolerance to abiotic and biotic stress. The HCAA clovamide is reported in a small number of distantly related species. To explore the contribution of specialized metabolites to disease resistance in cacao (Theobroma cacao L., chocolate tree), we performed untargeted metabolomics using liquid chromatography – tandem mass spectrometry (LC-MS/MS) and compared the basal metabolite profiles in leaves of two cacao genotypes with contrasting levels of susceptibility to Phytophthora spp. Leaves of the tolerant genotype ‘Scavina 6’ were found to accumulate dramatically higher levels of clovamide and several other HCAAs compared to the susceptible ‘Imperial College Selection 1’ (‘ICS1’). Clovamide was the most abundant metabolite in ‘Scavina 6’ leaf extracts based on MS signal, and was up to 58-fold higher in ‘Scavina 6’ than in ‘ICS1’. In vitro assays demonstrated that clovamide inhibits growth of three pathogens of cacao in the genus Phytophthora, is a substrate for cacao polyphenol oxidase, and is a contributor to enzymatic browning. Furthermore, clovamide inhibited proteinase and pectinase in vitro, activities associated with defense in plant-pathogen interactions. Fruit epidermal peels from both genotypes contained substantial amounts of clovamide, but two sulfated HCAAs were present at high abundance exclusively in ‘Scavina 6’ suggesting a potential functional role of these compounds. The potential to breed cacao with increased HCAAs for improved agricultural performance is discussed.
2020-12-17 | MTBLS2154 | MetaboLights
Project description:Inoculation of endophyte-free (E-) Theobroma cacao leaves with Colletotrichum tropicale (E+), the dominant foliar fungal endophyte in healthy T. cacao, induced significant changes in the expression of hundreds of host genes. Further, E+ leaves exhibit enhanced pathogen resistance, increased lignin and cellulose content, reduced maximum rates of photosynthesis (Amax), and enrichment of nitrogen-15 and carbon-13 isotopes that all correspond to the changes in expression of specific functional genes in related pathways. Moreover, a cacao gene highly up-regulated in E+ leaves increases pathogen resistance apart from any direct endophyte effects. Thus, benefits of increased pathogen resistance in E+ plants are partially due to enhanced induction of intrinsic host defense pathways, and potential costs include reduced photosynthetic capacity and endophyte metabolism of host tissues. Similar effects are likely to be properties of most plant-endophyte interactions, suggesting general relevance to the design and interpretation of studies of genetic and phenotypic expression in plants. This was a time course experiment, which objective was to assess gene expression changes in leaves of Theobroma cacao due to inoculation with endophyte Colletotrichum tropicale, over a period of two weeks. Three un-inoculated T. cacao leaves (E-) were collected at time 0, just prior to inoculation, and four T. cacao leaves inoculated with C. tropicale (E+) were collected at 3, 7, and 14 dpi, each leaf from a different plant. Sampled leaves were of developmental stage C-D (MejM-CM--a et al. 2012). MejM-CM--a, L.C., Guiltinan, M., J. , Shi, Z., Landherr, L., and Maximova, S., N. (2012). Expression of Designed Antimicrobial Peptides in Theobroma cacao L. Trees Reduces Leaf Necrosis Caused by Phytophthora spp, in Small Wonders: Peptides for Disease Control. American Chemical Society), 379-395.
2014-08-12 | E-GEOD-60357 | biostudies-arrayexpress
Project description:Population genomic analyses provide insights on population structure and conservation strategies of incense tree Aquilaria sinensis
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