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Exome sequencing of human ex vivo ovarian cancer models with matched stromal cells

ABSTRACT: Exome sequencing of human ex vivo ovarian cancer models with matched stromal cells

PROVIDER: PRJEB28710 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR2808290_1.fastq.gz	Fastqsanger.gz
	ERR2808290_2.fastq.gz	Fastqsanger.gz
	ERR2808300_1.fastq.gz	Fastqsanger.gz
	ERR2808300_2.fastq.gz	Fastqsanger.gz
	ERR2808310_1.fastq.gz	Fastqsanger.gz

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Exome sequencing of human ex vivo ovarian cancer models with matched stromal cells

Project description:Ascites or solid tumour from patients with ovarian cancer was collected and grown in culture as ex vivo models. Each sample has a mixture of tumour and stromal cells which were separated into individual cultures. Therefore each patient has tumour and stromal cultures originating from the same tissue collection. Variant calling (exome-seq) analysis was performed on these matched models to establish tumour specific mutations. Stromal cells were used to rule out germline mutations.

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Project description:Ascites or solid tumour samples from patients with ovarian cancer were collected and grown in culture as ex vivo models of purified tumour cells. RNA-seq was performed on these models to establish gene expression profiles, which allow identification of genes that are differentially expressed between patients with differing tumour intrinsic properties. These samples have been interrogated for the presence of a gene expression signature indicative of sensitivity to an inhibitor of poly(ADP-ribose) glycohydrolase (PARG). These samples are processed in the same manner as previous studies: “E-MTAB-7223 - RNA-seq of human ex vivo ovarian cancer models with matched stromal cells” and “E-MTAB-10801 - RNA-seq of human ex vivo ovarian cancer models with matched stromal cells - part II” with no stromal counterparts included in this current sequencing batch.

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