Project description:In this study, we recruited a patient with Hereditary spherocytosis (HS) detected to have a novel heterozygous variant in the SPTB in the proband. Sanger sequencing of variant alleles and haplotype linkage analysis were performed simultaneously. Five embryos were identified with one heterozygous and four not carrying the SPTB variant. Single-cell amplification and whole genome sequencing showed that three embryos had varying degrees of trisomy mosaicism.
Project description:In this research, capillary electrophoresis was combined with liquid chromatography to sequence novel monoclonal antibodies. It was demonstrated CE can provide highly complemetary information to LC in the process of novel mAbs sequencing.
