Project description:Whole human genome sequencing of an CEPH/UTAH female individual (HapMap: NA12878) using the Illumina HiSeq 2000 and paired 100 base reads
| PRJEB2890 | ENA
Project description:Whole genome sequencing of human HapMap individual NA12877 using the Illumina HiSeq 2000 and paired 100 base reads
| PRJEB2889 | ENA
Project description:Whole human genome sequencing of an African male individual (HapMap: NA18507) using the Illumina HiSeq 2500 and paired 100 base reads
Project description:We used microarrays to examine gene expression levels from 131 unrelated CEPH-Utah grandparents with either DMSO or tunicamycin. We measured gene expression levels in immortalized B cells from 131 unrelated CEPH-Utah grandparents. Each individual was treated for 8 hours with either DMSO or with 4 ug/ml of tunicamycin. Gene expression was measured.
Project description:Alternative pre-mRNA splicing increases proteomic diversity and provides a potential mechanism underlying both phenotypic diversity and susceptibility to genetic disorders in human populations. To investigate the variation in splicing among humans on a genomewide scale, we use a comprehensive exon-targeted microarray to examine alternative splicing in lymphoblasts derived from the CEPH HapMap population. We show the identification of transcripts containing annotated and novel sequence verified exon skipping, intron retention, and cryptic splice site usage that are specific between individuals. Using family-based linkage analysis, we demonstrate Mendelian inheritance and segregation of specific splice isoforms with regulatory haplotypes for three genes. Allelic association was further used to identify individual SNPs or regulatory haplotype blocks linked to the alternative splicing event, taking advantage of the high-resolution genotype information from the CEPH HapMap population. Keywords: Comparative genomic hybridiation within a 3 generation pedigree We used 14 individuals from the 3 generation CEPH/UTAH 1444 pedigree for our analysis of looking at heritability of differentially spliced exons within the family. 3 biological growths of individuals NA12739, NA12740, NA12750, and NA12751 were used and a single biological growth for the remaining 10 individuals.
Project description:We used microarrays to examine gene expression levels from 95 unrelated CEPH-Utah individuals 0, 2 or 6 hours after treatment with 10Gy of ionizing radiation. We measured gene expression levels in immortalized B cells from 95 unrelated CEPH-Utah individuals. Each individual was treated with 10Gy of ionizing radiation and expression was measured 0, 2 and 6 hour after treatment.
Project description:Whole human genome sequencing of an African female individual (HapMap: NA18508) using the Illumina Genome Analyzer IIx and paired 100 base reads
Project description:Whole human genome sequencing of an African male individual (HapMap: NA18506) using the Illumina Genome Analyzer IIx and paired 100 base reads
Project description:Whole human genome sequencing of an African male individual (HapMap: NA18507) using the Illumina Genome Analyzer IIx and paired 100 base reads
