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Ancient Iberian genomes

ABSTRACT: This study includes mitogenomes and whole nuclear genomes from prehistoric Iberian remains

PROVIDER: PRJEB29189 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR2843830.fastq.gz	Fastqsanger.gz
	ERR2843840.fastq.gz	Fastqsanger.gz
	ERR2843831.fastq.gz	Fastqsanger.gz
	ERR2843832.fastq.gz	Fastqsanger.gz
	ERR2843833.fastq.gz	Fastqsanger.gz

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Project description:The hypothalamus is implicated in controlling feeding and adiposity, besides many other physiological functions, and thus can be of great importance in explaining productive differences between lean and fatty pig breeds. The present study aimed to evaluate the hypothalamic transcriptome of pure Iberian (IBxIB) and Large White x Iberian crossbreds (IBxLW) at 60 days-old, produced in a single maternal environment. Results showed the implication of gender and genotype in the hypothalamic transcriptome, with 51 differentially expressed genes (DEGs) between genotypes and 10 DEGs between genders. Fourteen genotype by sex interactions were found, due to a higher genotype effect on transcriptome found in males. In fact, just 31 DEGs were identified when using only females but 158 using only males. A higher expression of genes related to mitochondrial activity in IBxIB male animals (ND3, ND4, ND5, UQCRC2 and ATP6) was found, which was related to a higher oxidative phosphorylation and greater reactive oxygen species and nitric oxide production. IBxLW male animals showed higher expression of SIRT3 regulator, also related to mitochondrial function. When females were analysed, no such differences were found, since only some differences in genes related to the tricitric acid cycle were observed. Thus, the results indicate a significant effect and interaction of the breed and the sex on the hypothalamic transcriptome at this early age.

2022-04-28 | GSE201408 | GEO

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