A missense ENU mutation in choline acetyltransferase
Ontology highlight
ABSTRACT:
PROVIDER: PRJEB29601 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| ERR2886550_1.fastq.gz | Fastqsanger.gz | |||
| ERR2886550_2.fastq.gz | Fastqsanger.gz | |||
| ERR2886551_1.fastq.gz | Fastqsanger.gz | |||
| ERR2886551_2.fastq.gz | Fastqsanger.gz |
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Project description:We use ChAT(BAC)-EGFP mice, which express enhanced green fluorescent protein (EGFP) under the control of transcriptional regulatory elements for choline acetyltransferase (ChAT), the sole enzyme that catalyzes the biosynthesis of acetylcholine (ACh). These mice were treated with inducers of ChAT. Kidney were rapidly removed, frozen on liquid nitrogen and stored at -80C
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Project description:The E1A binding protein P300 (EP300, also known as p300, lysine acetyltransferase 3B or KAT3B) and its close paralogue CREB-binding protein (CREBBP, aka CBP or KAT3A) possess intrinsic histone acetyltransferase (HAT) activity that can act on both histone and non-histone proteins. P300 and CBP are composed of multiple conserved protein domains, many of which are in proximity to the HAT domain modulating its catalytic activity. Here we show that the TAZ2 domain regulates the intrinsic catalytic activity of p300 in vitro and histone acetylation and chromatin accessibility in cells. Our study extends the knowledge of p300 self-regulation and provides new therapeutic stratagies for human cancers with corresponding p300/CBP mutations.
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