Project description:Microbial Electrochemical Technologies are based on the use of electrochemically active microorganisms that can carry out extracellular electron transfer to an electrode while they are oxidizing the organic compounds. The dynamics and changes of the bacterial community in the anode biofilm and planktonic broth of an acetate fed batch single chamber air cathode MFC have been studied by combing flow-cytometry and Illumina sequencing techniques. At the beginning of the test, from 0?h to 70?h, microbial planktonic communities changed from four groups to two groups, as revealed by DNA content, and from three groups to one group based on the cell membrane polarization revealed by a DiOC6(3) probe. Between 4th day and 13th day, microbial communities changed from one group to a maximum of three groups, monitoring DNA content, and from one group to two based on the cell membrane polarization. The 16S rDNA gene profiling confirmed the shift in microbial communities, with Acinetobacter (39.34%), Azospirillum (27.66%), Arcobacter (4.17%) and Comamonas (2.62%) being the most abundant genera at the beginning of MFC activation. After 70?h the main genera detected were Azospirillum (46.42%), Acinetobacter (34.66%), Enterococcus (2.32%), Dysgonomonas (2.14%). Data obtained have shown that flow cytometry and illumina sequencing are useful tools to monitor "online" the changes in microbial communities during the MFCs start-up and the increase of Azospirillum and Acinetobacter genera is in good agreement with the MFC voltage generation. Moreover, monitoring planktonic populations, instead of the less accessible anode biofilm, was in good agreement with the evolution of MFC voltage.
Project description:Monitoring microbial communities can aid in understanding the state of these habitats. Environmental DNA (eDNA) techniques provide efficient and comprehensive monitoring by capturing broader diversity. Besides structural profiling, eDNA methods allow the study of functional profiles, encompassing the genes within the microbial community. In this study, three methodologies were compared for functional profiling of microbial communities in estuarine and coastal sites in the Bay of Biscay. The methodologies included inference from 16S metabarcoding data using Tax4Fun, GeoChip microarrays, and shotgun metagenomics.
Project description:Microfluidic microbial fuel cells (MMFCs) are promising green power sources for future ultra-small electronic devices. The MMFCs with co-laminar microfluidic structure are superior to other MMFCs according to their low internal resistance and relative high power density. However, the area for interfacial electron transfer between the bacteria and the anode is quite limited in the typical Y-shaped device, which apparently restricts the current generation performance. In this study, we developed a membraneless MMFC with serpentine microchannel to enhance the interfacial electron transfer and promote the power generation of the device. Owing to the merit of laminar flow, the proposed MMFC was working well without any proton exchange membrane (PEM). At the same time, the serpentine microchannel greatly increased the power density. The S-MMFC catalyzed by Shewanella putrefaciens CN32 achieves a peak power density of 360 mW/m2 with the optimal channel configuration and the flow rate of 5 ml/h. Meanwhile, this device possesses much shorter start-up time and much longer duration time at high current plateau than the previous reported MMFCs. The presented MMFC appears promising for biochip technology and extends the scope of microfluidic energy.