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Intestinal commensal bacteria in tree shrew

ABSTRACT: In this study, we isolated several intestinal bacteria from tree shrew, such as Proteus spp. and E. coli. We performed the whole genomic sequencing for some typical strains, and wanted to find out the characteristics of these bacteria.

PROVIDER: PRJEB30582 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR3040170_1.fastq.gz	Fastqsanger.gz
	ERR3040170_2.fastq.gz	Fastqsanger.gz
	ERR3040180_1.fastq.gz	Fastqsanger.gz
	ERR3040180_2.fastq.gz	Fastqsanger.gz
	ERR3040190_1.fastq.gz	Fastqsanger.gz

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Project description:BackgroundTree shrew is a novel laboratory animal with specific characters for human disease researches in recent years. However, little is known about its characteristics of gut microbial community and intestinal commensal bacteria. In this study, 16S rRNA sequencing method was used to illustrate the gut microbiota structure and commensal Enterobacteriaceae bacteria were isolated to demonstrate their features.ResultsThe results showed Epsilonbacteraeota (30%), Proteobacteria (25%), Firmicutes (19%), Fusobacteria (13%), and Bacteroidetes (8%) were the most abundant phyla in the gut of tree shrew. Campylobacteria, Campylobacterales, Helicobacteraceae and Helicobacter were the predominant abundance for class, order, family and genus levels respectively. The alpha diversity analysis showed statistical significance (P < 0.05) for operational taxonomic units (OTUs), the richness estimates, and diversity indices for age groups of tree shrew. Beta diversity revealed the significant difference (P < 0.05) between age groups, which showed high abundance of Epsilonbacteraeota and Spirochaetes in infant group, Proteobacteria in young group, Fusobacteria in middle group, and Firmicutes in senile group. The diversity of microbial community was increased followed by the aging process of this animal. 16S rRNA gene functional prediction indicated that highly hot spots for infectious diseases, and neurodegenerative diseases in low age group of tree shrew (infant and young). The most isolated commensal Enterobacteriaceae bacteria from tree shrew were Proteus spp. (67%) and Escherichia coli (25%). Among these strains, the antibiotic resistant isolates were commonly found, and pulsed-field gel electrophoresis (PFGE) results of Proteus spp. indicated a high degree of similarity between isolates in the same age group, which was not observed for other bacteria.ConclusionsIn general, this study made understandings of the gut community structure and diversity of tree shrew.

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Intestinal commensal bacteria in tree shrew

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