Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Origin and differentiation trajectory of fibroblastic reticular cells in splenic white pulps - total splenic stroma cells - single cell RNA-seq

ABSTRACT: Origin and differentiation trajectory of fibroblastic reticular cells in splenic white pulps - total splenic stroma cells - single cell RNA-seq

PROVIDER: PRJEB31366 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Fibroblastic reticular cells in the spleen - total splenic Stroma cells - single cell RNAseq dataset 2

Project description:Splenic white pulp (WP) structures are underpinned by fibroblastic stromal cells (FSCs) to facilitate splenic compartmentalization and execute efficient immune responses. Although distinct WP FSCs exhibit various molecular traits, the origin and the hierarchical differentiation of different cell subsets are not characterized. Here we showed, the organization of splenic WP and the differentiation of WP FSCs were governed by lymphotoxin beta receptor (LTβR) signaling pathway. Cell fate mapping analysis revealed that different WP fibroblastic stromal cells descend from a common perivascular LTβR-sensitive mesenchymal lymphoid organizer cells (mLTo) at prenatal stage. Moreover, embryonic mLTo cells required LTβR signaling to give rise to different WP stromal cell subsets, while the proliferation of these cells was devoid of LTβR signaling but followed the development of WP during ontogeny. Moreover, cell fate mapping from different time point indicated a consecutive commitment of mLTo cells initiated from the proximal region around the splenic artery. RNAseq and differentiation trajectory analysis of distinct FSCs showed that Ltbr-deficient cells and perivascular reticular cells (PRCs) from adult spleen exhibited a progenitor phenotype and revealed a closer hierarchical lineage with embryonic mLTo cells. Taken together, our results unveil that embryonic mLTo cells residing in the perivascular niches can give rise to different FSC populations in a LTβR-dependent manner during development.

2019-03-27 | E-MTAB-7703 | biostudies-arrayexpress

Origin and differentiation trajectories of fibroblastic reticular cells in the splenic white pulp - single cell RNAseq dataset 1

2019-03-27 | E-MTAB-7094 | biostudies-arrayexpress

Fibroblastic reticular cells in the spleen - bulk RNAseq

2019-03-27 | E-MTAB-7097 | biostudies-arrayexpress

Bulk RNA-seq of Fibroblastic Reticular Cells

Project description:Fibroblastic reticular cells (FRCs) (CD45- Ter119-MADCAM1- CD21/35- CD31- PDPN-) from skin draining lymph nodes of Grem1-Cre-ERT2.cki; Rosa26-LSL-EYFP mice were sorted and subjected to bulk RNA-seq of Grem1 reporter + and Grem1 reporter - cells.

2021-04-10 | E-MTAB-10205 | biostudies-arrayexpress

Bulk RNA-seq of Fibroblastic Reticular Cells

Project description:Bulk RNA-seq of Fibroblastic Reticular Cells

| PRJEB43610 | ENA

RNA sequencing of splenic reticular cells in healthy and LCMV-infected mice

Project description:We report the transcriptome of subsets of mouse splenic mesenchymal reticular cells and lymph node reticular cells obtained from healthy Ccl19-cre x R26R-EYFP mice on a C57BL/6 background. We also examine splenic reticular cells from these mice infected with lymphocytic choriomeningitis virus (LCMV) strain Armstrong (acute infection) or strain Clone 13 (chronic infection). Subsets of CD45- CD31- Ter119- reticular cells were analyzed including PDPN+ T zone reticular cells (TRC) and MAdCAM-1+ marginal reticular cells (MRC) from spleen and lymph nodes. Additionally from the spleen we analyzed PDPN+ CD140a- cells, PDPN+ EYFP- cells, and EYFP+ red pulp reticular cells (RPRC) in healthy mice. We analyzed TRC, MRC, PDPN+ EYFP- cells, and EYFP+ RPRC from mice infected with LCMV Armstrong or LCMV Clone-13 on day 8 or on day 30 after infection. Our study represents the first detailed analysis of splenic reticular cell transcriptomes in healthy and LCMV-infected mice, with biologic replicates, generated by RNA-sequencing. We find substantial differential gene expression across subsets of splenic and lymph node reticular cells. We reveal significant changes in gene expression induced after virus infection that differ markedly with disease.

2022-01-09 | GSE171124 | GEO

Origin and differentiation trajectories of fibroblastic reticular cells in the splenic white pulp - bulk RNAseq

Project description:Origin and differentiation trajectories of fibroblastic reticular cells in the splenic white pulp - bulk RNAseq

| PRJEB28026 | ENA

Activation of TLR9 signaling suppresses the immuno modulating functions of CD55lo fibroblastic reticular cells during bacterial peritonitis

Project description:Fibroblastic reticular cells (FRCs) are heterogeneous. We use single cell RNA sequencing to identify subsets of FRCs in fat-associated lymphoid clusters (FALC) in the mesenteric adipose tissue

2024-04-18 | GSE250571 | GEO

Origin and differentiation trajectories of fibroblastic reticular cells in the splenic white pulp - single cell RNAseq

Project description:Origin and differentiation trajectories of fibroblastic reticular cells in the splenic white pulp - single cell RNAseq

| PRJEB28037 | ENA

bulk mRNA sequencing of splenic Red Pulp Fibroblasts (RPF) and lymph node Fibroblastic Reticular Cells (FRC)

Project description:bulk mRNA sequencing of splenic Red Pulp Fibroblasts (RPF) and lymph node Fibroblastic Reticular Cells (FRC)

| PRJNA624045 | ENA