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ChIP-seq against the CTCF protein in Raji cells with inducible BZLF1 protein (KF717093.1) prior to and post induction of BZLF1.

ABSTRACT: ChIP-seq against the CTCF protein in Raji cells with inducible BZLF1 protein (KF717093.1) prior to and post induction of BZLF1.

PROVIDER: PRJEB31753 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR3225425.fastq.gz	Fastqsanger.gz
	ERR3225426.fastq.gz	Fastqsanger.gz
	ERR3225427.fastq.gz	Fastqsanger.gz
	ERR3225428.fastq.gz	Fastqsanger.gz

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RNA-seq in Raji cells with inducible BZLF1 prior to and after induction of EBV's lytic cycle by doxycycline

Project description:(i) RNA transcripts of Raji iBZLF1 cells (https://www.biorxiv.org/content/10.1101/573659v1) were compared between non-induced cells and cells induced with doxycycline (100 ng/ ml) for 6 h. Human (Hg19) as well as viral (EBV, Raji KF717093.1) transcripts were analyzed. Artificial ERCC Spike-ins (Thermo Scientific) were added to selected samples to control for global changes of RNA levels. (ii) For control purposes, the RNA transcripts in Raji cells equipped with a doxycycline-controlled truncated BZLF1 allele lacking its transactivation domain (Raji iBZLF1 AD-truncated) were prior to and after induction for 6 h. (iii) For another control, the RNA transcripts of parental, unmodified Raji cells were analyzed prior to and after doxycycline induction for 6 h. All experiments were performed as triplicates.

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ChIP-seq against BZLF1 in Raji cells (EBV, Human Herpesvirus 4, GenBank accession KF717093).

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ChIP-seq analysis with an antibody directed against the H3K27me3 protein in Raji cells (Genbank: KF717093.1) prior to and post induction of BZLF1.

Project description:ChIP-seq analysis with an antibody directed against the H3K27me3 protein in Raji cells (Genbank: KF717093.1) prior to and post induction of BZLF1.

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ChIP-seq against the CTCF protein in Raji cells with inducible BZLF1 protein (KF717093.1) prior to and post induction of BZLF1.

Project description:Peaks were identified in both the human and viral (EBV (Human Herpesvirus 4; GenBank accession KF717093.1) genome.

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ChIP-seq analysis with an antibody directed against the human H3K4me3 protein in Raji cells (Genbank: KF717093.1) prior to and post induction of BZLF1.

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ChIP-seq analysis with an antibody directed against the human Rad21 protein in Raji cells (Genbank: KF717093.1) prior to and post induction of BZLF1.

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ChIP-seq analysis with an antibody directed against the human SMC1 protein in Raji cells (Genbank: KF717093.1) prior to and post induction of BZLF1

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ChIP-seq analysis with an antibody directed against the human H3K27ac protein in Raji cells (Genbank: KF717093.1) prior to and post induction of BZLF1.

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Project description:ChIP-seq analysis with an antibody directed against the human H3K4me1 protein in Raji cells (Genbank: KF717093.1) prior to and post induction of BZLF1.

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