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Cultivation of bacteria from Aplysina aerophoba

ABSTRACT: Cultivation of bacteria from Aplysina aerophoba: effects of oxygen and nutrient gradients

PROVIDER: PRJEB31820 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR3239030_1.fastq.gz	Fastqsanger.gz
	ERR3239030_2.fastq.gz	Fastqsanger.gz
	ERR3239040_1.fastq.gz	Fastqsanger.gz
	ERR3239040_2.fastq.gz	Fastqsanger.gz
	ERR3239050.fastq.gz	Fastqsanger.gz

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Cultivation of Bacteria From Aplysina aerophoba: Effects of Oxygen and Nutrient Gradients.

Project description:Sponge-associated bacteria possess biotechnologically interesting properties but as yet have largely evaded cultivation. Thus, "omics"-based information on the ecology and functional potential of sponge symbionts is awaiting its integration into the design of innovative cultivation approaches. To cultivate bacteria derived from the marine sponge Aplysina aerophoba, nine novel media formulations were created based on the predicted genomic potential of the prevalent sponge symbiont lineage Poribacteria. In addition, to maintain potential microbial metabolic interactions in vitro, a Liquid-Solid cultivation approach and a Winogradsky-column approach were applied. The vast majority of microorganisms in the inoculum appeared viable after cryopreservation of sponge specimen as determined by selective propidium monoazide DNA modification of membrane-compromised cells, however, only 2% of the initial prokaryotic diversity could be recovered through cultivation. In total, 256 OTUs encompassing seven prokaryotic phyla were cultivated. The diversity of the cultivated community was influenced by the addition of the antibiotic aeroplysinin-1 as well as by medium dilution, rather than carbon source. Furthermore, the Winogradsky-column approach reproducibly enriched distinct communities at different column depths, amongst which were numerous Clostridia and OTUs that could not be assigned to a known phylum. While some bacterial taxa such as Pseudovibrio and Ruegeria were recovered from nearly all applied cultivation conditions, others such as Bacteroidetes were specific to certain medium types. Predominant sponge-associated prokaryotic taxa remained uncultured, nonetheless, alternative cultivation approaches applied here enriched for previously uncultivated microbes.

| S-EPMC7042410 | biostudies-literature

Genomic collection of Actinobacteria associated with Aplysina aerophoba

Project description:Genomic collection of Actinobacteria associated with Aplysina aerophoba

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Aplysina aerophoba isolate:Ligurian sea | breed:marine sponge

Project description:Aplysina aerophoba isolate:Ligurian sea | breed:marine sponge Genome sequencing

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Transcriptomic profiles of the Mediterranean sponge species Aplysina aerophoba upon wounding by the specialist grazer Tylodina perversa

Project description:In the seabed, chemical defences mediate inter- and intraspecific interactions and may determine organisms’ success, shaping the diversity and function of benthic communities. Sponges represent a prominent example of chemically-defended marine organisms with great ecological success. The ecological factors controlling the production of their defensive compounds and the evolutionary forces that select for these defences remain little understood. Each sponge species produces a specific and diverse chemical arsenal with fish-deterrent, antifouling and antimicrobial properties. However, some small animals (mesograzers), mainly sea slugs, have specialized in living and feeding on sponges. Feeding on chemically-defended organisms provides a strategy to avoid predators, albeit the poor nutritional value of sponges. In order to investigate the mechanisms that control sponge chemical defence, with particular focus on the response to specialist grazers, we investigated the interaction between the sponge Aplysina aerophoba and the sea slug Tylodina perversa. Here we performed controlled experiments and collected sponge samples at different time points (3h, 1d and 6d after treatment). To further elucidate if the sponge response is specific to grazing by T. perversa, we also included a treatment in which sponges were mechanically damaged with a scalpel. We compared gene expression between treatments based on RNA-Seq data.

2022-01-30 | E-MTAB-8601 | biostudies-arrayexpress

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