Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Genome sequencing and variability of HSV-1 SC16 and HSV-2 333 strains

ABSTRACT: Genome sequencing and variability of HSV-1 SC16 and HSV-2 333 strains

PROVIDER: PRJEB32133 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	ERR3277140_1.fastq.gz	Fastqsanger.gz
	ERR3277140_2.fastq.gz	Fastqsanger.gz
	ERR3277150_1.fastq.gz	Fastqsanger.gz
	ERR3277150_2.fastq.gz	Fastqsanger.gz
	ERR3277141_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 32

Similar Datasets

HSV-2 strain 333 genome sequencing using PacBio

Project description:HSV-2 strain 333 genome sequencing using PacBio

| PRJEB40057 | ENA

Generation and sequencing of HSV genomic variability in cell culture.

Project description:Generation and sequencing of HSV genomic variability in cell culture.

| PRJEB32148 | ENA

Bradyrhizobium yuanmingense strain:USDA 333 | isolate:USDA 333

Project description:Bradyrhizobium yuanmingense USDA 333 genome sequencing

| PRJNA1115322 | ENA

Escherichia coli strain:333

Project description:Escherichia coli 333 genome sequencing

| PRJNA442000 | ENA

Phocaeicola coprophilus CAG:333

Project description:Bacteroides coprophilus CAG:333 overview

| PRJNA207262 | ENA

Vibrio campbellii CAIM 333

Project description:Vibrio campbellii CAIM 333 Genome sequencing

| PRJNA169678 | ENA

Wide-spread disruption of transcription termination in HSV-1 infection: Next generation sequencing of total and newly transcribed (4sU-RNA) RNA from different virus strains and mutant viruses

Project description:Primary human foreskin fibroblasts (HFF) were infected with the indicated HSV-1 strains and mutants thereof at a multiplicity of infection (MOI) of 10. Newly transcribed RNA was labelled by adding 500µM 4-thiouridine (4sU) to the cell culture media for 1h. Total cellular RNA was isolated using Trizol. Newly transcribed RNA was purified following the protocol described in Raedle et al. JoVE 2013.

2020-06-08 | GSE151912 | GEO

Sequencing of HSV-1 genomic DNA to identify genomic variation resulting from selective pressure imposed by mutation of the PP1 binding site in the pUL21 gene

Project description:The pUL21 gene is essential for efficient replication and dissemination in herpes simplex virus 1 (HSV-1) but the molecular basis for its function is not well understood. Experimental work revealed that pUL21 is a virus-encoded adaptor of protein phosphatase 1 (PP1). Mutation of the specific motif in pUL21 responsible for PPI recruitment, followed by propagation in cell culture, was performed in order to identify the impact of preventing PP1 recruitment on the viral genome. This allowed identification of compensatory mutations disrupting the viral kinase gene pUS3, which restored viral growth and spread, revealing that pUL21 directly antagonises pUS3. Three mutant strains of HSV-1 were generated, mutating the conserved residues F242 and V243 in the PP1 binding sequence, individually and in combination (pUL21F242E, pUL21V243D, pUL21FV242AA), along with a knockout strain lacking pUL21 (ΔpUL21). These four virus stocks, plus wild-type HSV-1, were propagated in Vero cells for three passages. Genomic DNA was extracted from the P3 viruses and sequenced with Illumina. Reads were trimmed and mapped to an edited version of the HSV-1 genome (based on JQ673480.1) and read depth and variants were quantified.

2021-07-31 | E-MTAB-10788 | biostudies-arrayexpress

Trichoderma virens FT-333

Project description:Trichoderma virens FT-333 Genome sequencing and assembly

| PRJNA268050 | ENA

Oryza sativa strain:ubi-XA21 FN-333 | cultivar:Kitaake ubi-XA21 FN-333

Project description:Oryza sativa strain:ubi-XA21 FN-333 | cultivar:Kitaake ubi-XA21 FN-333 Genome sequencing

| PRJNA333202 | ENA