Project description:BACKGROUND:Microbial communities present in environmental waters constitute a reservoir for antibiotic-resistant pathogens that impact human health. For this reason, a diverse variety of water environments are being analyzed using metagenomics to uncover public health threats. However, the composition of these communities along the coastal environment of a whole city, where sewage and beach waters are mixed, is poorly understood. RESULTS:We shotgun-sequenced 20 coastal areas from the city of Montevideo (capital of Uruguay) including beach and sewage water samples to characterize bacterial communities and their virulence and antibiotic resistance repertories. As expected, we found that sewage and beach environments present significantly different bacterial communities. This baseline allowed us to detect a higher prevalence and a more diverse repertory of virulence and antibiotic-resistant genes in sewage samples. Many of these genes come from well-known enterobacteria and represent carbapenemases and extended-spectrum betalactamases reported in hospital infections in Montevideo. Additionally, we were able to genotype the presence of both globally disseminated pathogenic clones and emerging antibiotic-resistant bacteria in sewage waters. CONCLUSIONS:Our study represents the first in using metagenomics to jointly analyze beaches and the sewage system from an entire city, allowing us to characterize antibiotic-resistant pathogens circulating in urban waters. The data generated in this initial study represent a baseline metagenomic exploration to guide future longitudinal (time-wise) studies, whose systematic implementation will provide useful epidemiological information to improve public health surveillance.

| S-EPMC6396544 | biostudies-literature

Caribbean coastal waters plastic discs 4 months

Project description:Caribbean coastal waters plastic discs 4 months Raw sequence reads

| PRJNA1054050 | ENA

Unicellular eukaryotes from Aegean Sea coastal waters

Project description:Unicellular eukaryotes from Aegean Sea coastal waters Raw sequence reads

| PRJNA515026 | ENA

Microbial communities in shallow coastal waters

Project description:Variability of nitrifying communities in surface coastal waters of the Eastern South Pacific (~36° S)

| PRJEB6968 | ENA

COVID-19 Genomics UK (COG-UK) consortium

Project description:COVID-19 Genomics UK (COG-UK) consortium

| PRJEB37886 | ENA

Transcription profiling of wild hornyhead turbot from polluted and clean coast waters to analyse endocrine disruption in southern Salifornia coastal fish

Project description:A custom multi-species microarray was used to study gene expression in wild hornyhead turbot (Pleuronichthys verticalis), collected from polluted and clean coastal waters in Southern California and in laboratory male zebrafish (Danio rerio) following exposure to estradiol and 4-nonylphenol. A multi-gene cross species microarray was fabricated as a diagnostic tool to screen the effects of environmental chemicals in fish, for which there is minimal genomic information. The microarray measurement of gene expression in zebrafish, which are phylogenetically distant from turbot, indicates that this multi-species microarray will be useful for measuring endocrine responses in Pleuronectiformes and other fish for which there is minimal genomic sequence information.

2009-07-17 | E-MTAB-43 | biostudies-arrayexpress

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