Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

N-valerate formation from methanol based propionate chain elongation in mixed cultures

ABSTRACT: N-valerate formation from methanol based propionate chain elongation in mixed cultures

PROVIDER: PRJEB32281 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	ERR3294580_1.fastq.gz	Fastqsanger.gz
	ERR3294580_2.fastq.gz	Fastqsanger.gz
	ERR3294590_1.fastq.gz	Fastqsanger.gz
	ERR3294590_2.fastq.gz	Fastqsanger.gz
	ERR3294581_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 24

Similar Datasets

chain elongation process

Project description:chain elongation process Genome sequencing and assembly

| PRJNA909000 | ENA

chain elongation process

Project description:chain elongation process with GAC Raw sequence reads

| PRJNA998485 | ENA

chain elongation process with FeO

Project description:chain elongation process with FeO Metagenome

| PRJNA1030079 | ENA

Methanol based chain elongation with acetate to n-butyrate and isobutyrate at varying selectivities dependent on pH

Project description:Methanol based chain elongation with acetate to n-butyrate and isobutyrate at varying selectivities dependent on pH

| PRJEB36205 | ENA

reactor microbiota of chain elongation with lactate

Project description:microbial chain elongation with lactate for producing medium-chain carboxylates

| PRJEB34417 | ENA

Gut-derived short-chain fatty acids are vividly assimilated into host carbohydrates and lipids

Project description:Acetate, propionate and butyrate are the main short-chain fatty acids (SCFAs) that arise from the fermentation of fibers by the colonic microbiota. While many studies focus on the regulatory role of SCFAs, their quantitative role as a catabolic or anabolic substrate for the host has received relatively little attention. To investigate this aspect, we infused conscious mice with physiological quantities of stable isotopes [1-13C]acetate, [2-13C]propionate or [2,4-13C2]butyrate directly into the cecum, which is the natural production site in mice, and analyzed their interconversion by the microbiota as well as their metabolism by the host. Cecal interconversion - pointing to microbial cross-feeding - was high between acetate and butyrate, low between butyrate and propionate and almost absent between acetate and propionate. As much as 62% of infused propionate was used in whole-body glucose production, in line with its role as gluconeogenic substrate. Conversely, glucose synthesis from propionate accounted for 69% of total glucose production. The synthesis of palmitate and cholesterol in the liver was high from cecal acetate (2.8% and 0.7%, respectively) and butyrate (2.7% and 0.9%, respectively) as substrates, but low or absent from propionate (0.6% and 0.0%, respectively). Label incorporation due to chain elongation of stearate was approximately 8-fold higher than de novo synthesis of stearate. Microarray data suggested that SCFAs exert only a mild regulatory effect on the expression of genes involved in hepatic metabolic pathways during the 6h infusion period. Altogether, gut-derived acetate, propionate and butyrate play important roles as substrates for glucose, cholesterol and lipid metabolism. Mice were infused in cecum with stably-labelled isotopes of the three main short chain fatty acids or control solution. After 6 hrs, livers were removed and pooled RNA samples were subjected to gene expression profiling.

2013-10-21 | E-GEOD-45926 | biostudies-arrayexpress

Short-chain fatty acids propionate and butyrate control growth and differentiation linked to cellular metabolism [RNA-Seq]

Project description:The short-chain fatty acids (SCFA) propionate and butyrate are produced in large amounts by microbial metabolism and have been identified as unique acyl lysine histone marks. In order to better understand the function of these modifications we used ChIP-Seq to map the genome-wide location of four short-chain acyl histone marks H3K18pr/bu and H4K12pr/bu in treated and untreated colorectal cancer (CRC) and normal cells, as well as in mouse intestines in vivo. We correlate these marks with open chromatin regions along with gene expression to access the function of the target regions. Our data demonstrate that propionate and butyrate act as promoters of growth, differentiation as well as ion transport. We propose a mechanism involving direct modification of specific genomic regions, resulting in increased chromatin accessibility, and in case of butyrate, opposing effects on the proliferation of normal versus CRC cells.

2024-01-10 | GSE252753 | GEO

Short-chain fatty acids propionate and butyrate control growth and differentiation linked to cellular metabolism [ATAC-Seq]

2024-01-10 | GSE252652 | GEO

Short-chain fatty acids propionate and butyrate control growth and differentiation linked to cellular metabolism [ChIP-Seq]

2024-01-10 | GSE252649 | GEO

Short-chain fatty acids propionate and butyrate control growth and differentiation linked to cellular metabolism [CUT&Tag]

2024-01-10 | GSE252754 | GEO