Project description:Sirturo or Bedaquiline has been shown to inhibit the ATP synthase of Mycobacterium tuberculosis. We used microarrays to investigate compound-induced gene expression changes in general as well as effects on the transcription of the different ATP synthase genes and other metabolic pathways. Log phase Mycobacterium tuberculosis were cultivated in Middlebrook 7H9 broth and treated with 1 M-BM-5M Bedaquiline. We have extracted RNA from five different time-points after treatment: 0 min (T0), 30 min (T30), 60 min (T60), 180 min (T180) and 360 min (T360).
Project description:GeneXpert-evading MDR M. tuberculosis outbreak clone in Eswatini has elevated bedaquiline and clofazimine resistance levels dated to the pre-treatment era
Project description:GeneXpert-evading MDR M. tuberculosis outbreak clone in Eswatini has elevated bedaquiline and clofazimine resistance levels dated to the pre-treatment era
Project description:Bacteria commonly adapt to stresses by altering gene expression. To understand the response of M. tuberculosis (MTB) to bedaquiline, we performed transcriptomics over a time-course on MTB bacilli exposed to the drug.
