Project description:Sirturo or Bedaquiline has been shown to inhibit the ATP synthase of Mycobacterium tuberculosis. We used microarrays to investigate compound-induced gene expression changes in general as well as effects on the transcription of the different ATP synthase genes and other metabolic pathways. Log phase Mycobacterium tuberculosis were cultivated in Middlebrook 7H9 broth and treated with 1 M-BM-5M Bedaquiline. We have extracted RNA from five different time-points after treatment: 0 min (T0), 30 min (T30), 60 min (T60), 180 min (T180) and 360 min (T360).
Project description:We profiled Mtb transcriptional response to a range of different antibiotic drug exposures in 7H9 broth culture by RNA-seq. Compound abbreviations: (AMK = amikacin, BDQ = bedaquiline, CPF = ciprofloxacin, LVF = levofloxacin, MXF = moxifloxacin, CFZ = clofazimine, PA824 = pretomanid, RPE = rifapentine, PZA = pyrazinamide). We also hypothesized that an improved Mycobacterium tuberculosis (Mtb) transcriptional regulatory network (TRN) could be inferred using a large, diverse compendium of RNA-seq data and therefore sought to supplement the existing repository of Mtb expression data available on SRA with additional conditions that could inform on Mtb's underlying regulatory programs. We went on to infer Mtb's TRN using a collection of inference methods in combination with this compendium, and employed it to build an interpretable machine learning model of Mtb fitness under stress.
2025-03-19 | GSE292332 | GEO
Project description:The role of epistasis in amikacin, kanamycin, bedaquiline, and clofazimine resistance in Mycobacterium tuberculosis complex
Project description:GeneXpert-evading MDR M. tuberculosis outbreak clone in Eswatini has elevated bedaquiline and clofazimine resistance levels dated to the pre-treatment era
Project description:GeneXpert-evading MDR M. tuberculosis outbreak clone in Eswatini has elevated bedaquiline and clofazimine resistance levels dated to the pre-treatment era
