Project description:Mongolia has the highest reported incidence of (and mortality from) hepatocellular carcinoma (HCC) in the world. This is the first molecular study aiming to characterize the genomic landscape of Mongolian HCC.
Project description:Objective: Detection and analysis of Mongolian medicine Narenmandula the kernel full of adriamycin nephrosis rats kidney tissue expression of miRNA. Methods: The biological information of the differentially expressed miRNA between the normal group, the model group and the Mongolian medicine Narenmandula group was studied by Affymetrix miRNA chip detection. Results: The biological information of miRNA expression in kidney tissues of rats with doxorubicin nephropathy was analyzed by detection, which laid a foundation for further elucidating the multi-target regulatory role of Mongolian medicine Narenmandula in renal function protection and provided data. The biological information of miRNA expression in renal tissues of doxorubicin rats was detected and analyzed using Affymetrix miRNA chip detection technology, which laid a foundation for further elucidating the multi-target regulation effect of the renal function protection and provided data of Mongolian medicine Narenmandula .
Project description:We recruited 24 Mongolian volunteers,6 of which were T2D cases(sample T1-T6), 6 were prediabetes cases(sample P1-P6), and 12 were health cases(sample C1-C12). The metagenomic analysis of gut microbiota from the volunteers’ fecal samples was performed. We compared the microbial differences in the three groups, and analyzed the differences of the stool microbial function.
Project description:The Mongolian horse has the characteristics of cold resistance, rough feeding resistance, good endurance, strong disease resistance and strong gregariousness. The normal production of sperm in male mammals is the basis of reproduction, and spermatogenesis includes a mitosis, meiosis twice and metamorphosis of sperm cells. The MEI1 gene is involved in the meiosis cycle and is required for normal meiosis chromosome association. Previous studies have shown that alternative splicing of MEI1 gene can promote spermatogenesis in Mongolian horses. In this study, the expression of MEI1 gene was determined by immunofluorescence in the third generation Mongolian horse testicular sertoli cells. Two overexpressed lentiviral vectors with MXE and SE events of MEI1 gene were constructed and successfully infected into sertoli cells. CCK-8 was used to detect the proliferation and activity of infected cells, transcripomics and metabolomics were used to screen and annotate differential genes and differential metabolites in the two sertoli cells with MXE and SE events. After 72 h of virus infection, 193 differentially expressed genes and 11360 differentially expressed metabolites were screened, 109 up-regulated genes including MEI1 and 84 down-regulated genes, 7494 up-regulated metabolites and 3866 down-regulated metabolites. Differential genes and differential metabolites were mainly enriched in several pathways related to spermatogenesis. Differential genes such as IL31RA, ATP2B3, CASQ2 were highly expressed in SE events, IL11, PRLR, CCR7 were highly expressed in MXE events. Metabolites such as folic acid and spermine were highly expressed in SE events, citric acid and glutathione were highly expressed in MXE events. This suggested that both MXE and SE events of MEI1 gene can promote the activity of spermatogenesis signaling pathway, and the gene influences spermatogenesis by regulating the expression of metabolites. In summary, MXE and SE events of MEI1 gene can promote the expression of spermatogen-related genes and metabolites, and genes affect spermatogenesis by regulating the expression of metabolites. The results of this study laid a theoretical foundation for exploring the regulatory mechanism of different alternative splicing events on spermatogenesis in Mongolian horse.
Project description:Mongolian gerbil (Meriones unguiculatus) is an ideal animal model for non-alcoholic fatty liver disease/nonalcoholic steatohepatitis (NAFLD/NASH) which is under development. Currently, the research and application of Mongolian gerbils have been largely limited due to the lack of genomic and transcriptome information. In the present study, we sought to better understand the underlying mechanism of the rapid development of NASH in high-fat diet (HFD)-fed Mongolian gerbils by analyzing the transcriptome profilings of different modeling times. Eight hub genes CD44, APP, CDC42, CD68, CXCR4, CSF1R, ADGRE1 and FERMT3 were obtained, which are all involved in inflammation response and CDC42 and FERMT3 are associated with hepatocellular carcinoma (HCC). Most of the hub genes showed high expression levels in the first stage of modeling-building (HFD for 4 weeks). We observed that feeding HFD for 4 weeks is sufficient to induce high expression of pro-fibrotic factors such as PDGFB and CTGF and to form collagen deposits. Intriguingly, we found four significant independent prognostic factors for HCC: GPC1, ARPC1B, DAB2 and CFL exhibiting high expression levels during the modeling time. Our findings indicated that, Mongolian gerbil is an ideal NAFLD model with rapid progression in inflammation and fibrosis. Prolonging the modeling time may lead to the formation of NAFLD-related HCC and further research is needed to investigate this possibility.
Project description:Lakes are widely distributed on the Mongolian Plateau and, as critical water sources, have sustained Mongolian pastures for hundreds of years. However, the plateau has experienced significant lake shrinkage and grassland degradation during the past several decades. To quantify the changes in all of the lakes on the plateau and the associated driving factors, we performed a satellite-based survey using multitemporal Landsat images from the 1970s to 2000s, combined with ground-based censuses. Our results document a rapid loss of lakes on the plateau in the past decades: the number of lakes with a water surface area >1 km(2) decreased from 785 in the late 1980s to 577 in 2010, with a greater rate of decrease (34.0%) in Inner Mongolia of China than in Mongolia (17.6%). This decrease has been particularly pronounced since the late 1990s in Inner Mongolia and the number of lakes >10 km(2) has declined by 30.0%. The statistical analyses suggested that in Mongolia precipitation was the dominant driver for the lake changes, and in Inner Mongolia coal mining was most important in its grassland area and irrigation was the leading factor in its cultivated area. The deterioration of lakes is expected to continue in the following decades not only because of changing climate but also increasing exploitation of underground mineral and groundwater resources on the plateau. To protect grasslands and the indigenous nomads, effective action is urgently required to save these valuable lakes from further deterioration.
Project description:The genetics, social, cultural and environmental factors pose a great challenge for the diagnosis and treatment of coronary heart disease among different racial groups. We aimed to identify the differentially expressed genes involved in coronary heart disease in Chinese Han people as an aid for screening and diagnosing coronary heart disease. We used microarrays to detail the global programme of gene expression to identify the differentially gene between the patients with coronary heart disease and healthy people in Chinese Han people Three patients with coronary heart disease and three healthy people in Chinese Han people were recruited,total RNA of each samples were extracted from peripheral blood to hybridize with Affymetrix microarrays.