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Analysis of the 3’-end of piRNAs in the COLO 205 cell line through sodium periodate (NaIO4) /β-Elimination treatment and small RNA-Seq

ABSTRACT: Analysis of the 3’-end of piRNAs in the COLO 205 cell line through sodium periodate (NaIO4) /β-Elimination treatment and small RNA-Seq

PROVIDER: PRJEB33349 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR3415700.fastq.gz	Fastqsanger.gz
	ERR3415701.fastq.gz	Fastqsanger.gz
	ERR3415702.fastq.gz	Fastqsanger.gz
	ERR3415703.fastq.gz	Fastqsanger.gz
	ERR3415704.fastq.gz	Fastqsanger.gz

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Analysis of the 3’-end of piRNAs in the COLO 205 cell line through sodium periodate (NaIO4) /β-Elimination treatment and small RNA-Seq

Project description:Using a chemical treatment, the sodium periodate (NaIO4) /β-Elimination oxidation, and then performing deep-sequencing of small RNAs with NGS technologies, we evaluated the methylation status of the piRNAs population in the metastatic COLO 205 cell line.

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RNA-Immunoprecipitation of PIWIL1/piRNA complexes in COLO 205 cells and deep-sequencing of associated small RNAs with NGS technologies

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Project description:WIND: A Workflow for pIRNAs aNd beyonD, for the evaluation of the performance in piRNAs high expression and low expression conditions, we used Human Testis RNAs (BioChain Institute Inc, Newark, CA, USA) and COLO 205 cell line RNAs (samples are available on ArrayExpress (E-MTAB-8115: Non_treated_Testis_1 and Non_treated_COLO205_1, Non_treated_COLO205_2, Non_treated_COLO205_3).

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Colo-205

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Transcriptome profiling of total RNA in the COLO 205 cell line with Next-Generation Sequencing (NGS) technologies

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