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Nine-spined stickleback genome

ABSTRACT: Genome sequencing of the nine-spined stickleback (Pungitius pungitius) provides insights into chromosome evolution

PROVIDER: PRJEB33823 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
		Other
	ERR3569182_subreads.fastq.gz	Fastqsanger.gz
	ERR3618123_1.fastq.gz	Fastqsanger.gz
	ERR3618123_2.fastq.gz	Fastqsanger.gz
	ERR3618124_1.fastq.gz	Fastqsanger.gz

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Introgression in Nine-spined Sticklebacks

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Project description:Adult female three-spined stickleback fish (Gasterosteus aculeatus) were exposed to 10 individual chemicals, 26 mixtures of these chemicals, or control conditions in a flow-through system for 4 days. Transcriptomics was performed on liver samples by microarray. The main aims were to determine molecular signatures induced by these chemicals in the three-spined stickleback, discover whether these persisted in chemical mixtures and identify non-additive molecular responses in chemical mixtures exposures.

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Subtle effects of radiation on embryo development of the 3-spined stickleback

Project description:Following serious accidents at the Chernobyl and Fukushima nuclear power plants in 1986 and 2011 respectively, local flora and fauna have been chronically exposed to environmental radiation for many years . However, little is known about the environmental effects of low doses of radiation on aquatic organisms. The present study assesses the effect of an environmentally relevant dose range of radiation (Control: 0, Low (L): 0.1, Medium (M): 1 and High (H): 10 mGy/day) of waterborne 32P on early life stages of the 3-spined stickleback, Gasterosteus aculeatus. Here we assess the global effects on gene regulation on the embryo development of fish suffering from differing levels of environmental radiation to identify significantly altered transcriptional networks as a result of sustained radiation exposure.

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Tapeworm infection affects sleep behavior in three-spined stickleback

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Tapeworm infection affects sleep behavior in three-spined stickleback

Project description:Sleep is a complex and conserved biological process that affects several body functions and behaviors. Evidence suggests that there is a reciprocal interaction between sleep and immunity. For instance, fragmented sleep can increase the probability of parasitic infections and reduce the ability to fight infections. Moreover, viral and bacterial infections alter the sleep patterns of infected individuals. However, the effects of macro-parasitic infections on sleep remain largely unknown. In this study, we investigated whether macro-parasite infections could alter the sleep of their hosts. We experimentally infected three-spined sticklebacks (Gasterosteus aculeatus) with the tapeworm Schistocephalus solidus and used a hidden Markov model to characterize sleep behavior in sticklebacks. One to four days after parasite exposure, infected fish showed no difference in sleep compared with non-exposed fish, whereas fish that were exposed-but-not-infected slept less during daytime. 29-32 days after exposure, infected fish slept more than uninfected fish, while exposed-but-not-infected fish slept less than non-exposed fish. Using brain transcriptomics, we identified immune- and sleep-associated genes that potentially underlie the observed behavioral changes. These results provide insights into the complex association between macro-parasite infection, immunity, and sleep in fish and may thus contribute to a better understanding of reciprocal interactions between sleep and immunity.

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Project description:nine-spined stickleback sequencing data

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Project description:In order to identify gene expression difference between marine and freshwater stickleback populations, we compared the transcriptomes of seven adult tissues (eye, gill, heart, hypothalumus, liver, pectoral muscle, telencephalon) between a marine population sampled from the mouth of the Little Campbell river in British Columbia (LITC) and a freshwater population (Fishtrap Creek, FTC) from northern Washington. For each population, the sampled individuals were the lab-reared progeny of a single pair of wild-caught parents.

2011-12-30 | GSE34783 | GEO

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