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Transcriptomic response of B16-F1 melanomas to stearoyl-coA desaturase inhibitor A939572 in vivo

ABSTRACT: Transcriptomic response characterized by 3'RNA-seq of B16-F1 melanomas upon short-term treatment with stearoyl-coA desaturase inhibitor A939572 in vivo

PROVIDER: PRJEB33932 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR3522040.fastq.gz	Fastqsanger.gz
	ERR3522041.fastq.gz	Fastqsanger.gz
	ERR3522042.fastq.gz	Fastqsanger.gz
	ERR3522043.fastq.gz	Fastqsanger.gz
	ERR3522044.fastq.gz	Fastqsanger.gz

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Stearoyl-CoA desaturase mediated monounsaturated fatty acid availability supports humoral immunity

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Stearoyl-CoA desaturase mediated monounsaturated fatty acid availability supports humoral immunity

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Project description:Stearoyl-CoA desaturase 1-deficient (SCD1-/-) mice have impaired monounsaturated fatty acid (MUFA) synthesis. When maintained on a very low-fat, high-carbohydrate (VLF-HC) diet, SCD1-/- mice develop severe hypercholesterolemia characterized by an increase in apolipoprotein B-containing lipoproteins and the appearance of lipoprotein-X. Additionally, high-density lipoprotein cholesterol is dramatically reduced in VLF-HC SCD1-/- mice. The concomitant presence of elevated plasma bile acids, bilirubin and aminotransferases in the VLF-HC SCD1-/- mouse are indicative of hepatic dysfunction. Supplementation of the VLF-HC diet with unsaturated fat (canola oil), but not saturated fat (coconut oil), prevents these plasma phenotypes. However, dietary oleate was not as effective as canola oil in reducing low-density lipoprotein cholesterol, signifying an additional role for dietary polyunsaturated fatty acid deficiency in the development of this phenotype. These results indicate that lack of SCD1 results in an increased requirement for dietary unsaturated fat to compensate for impaired MUFA synthesis and to prevent hypercholesterolemia and hepatic dysfunction. Experiment Overall Design: We used Affymetrix Mouse430v2.0 microarray chips to search for gene expression changes unique to the VLF-HC SCD1-/- group. These chips contain 45,101 probe sets representing over 39,000 transcripts and variants from over 34,000 mouse genes. We studied SCD1+/+ and SCD1-/- mice on chow or the VLF-HC diet, analyzing five individual livers from each subgroup for a total of twenty microarrays.

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