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ATAC-seq analyses with results from DG75 cells prior to and post induction of two versions of BZLF1.

ABSTRACT: ATAC-seq analyses with results from DG75 cells prior to and post induction of two versions of BZLF1.

PROVIDER: PRJEB34834 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR3588770.fastq.gz	Fastqsanger.gz
	ERR3588771.fastq.gz	Fastqsanger.gz
	ERR3588772.fastq.gz	Fastqsanger.gz
	ERR3588773.fastq.gz	Fastqsanger.gz
	ERR3588774.fastq.gz	Fastqsanger.gz

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ATAC-seq analyses with results from DG75 cells prior to and post induction of two versions of BZLF1.

Project description:The aim of the experiment was to identify accessible (open) chromatin regions in the genome prior to and post induction of EBV's BZLF1 protein. Two versions of BZLF1 were employed: full-length and activation-domain (AD)-truncated BZLF1 non-induced and induced for 15 h. The experiments were performed as triplicates.

2021-02-07 | E-MTAB-8420 | biostudies-arrayexpress

RNA-seq data from DG75 cells with inducible BZLF1 prior to and after induction of EBV's lytic cycle by doxycycline.

Project description:(i) Transcripts of DG75 iBZLF1 cells were analyzed comparing non-induced cells and cells induced with doxycycline (100 ng/ ml) for 6 h. Doxycycline induces the expression of the full length viral transcription factor BZLF1. (ii) For control purposes, the transcripts of DG75 cells equipped with a doxycycline-regulated truncated BZLF1 allele lacking its transactivation domain (DG75 iBZLF1 AD-truncated) were analyzed prior to and after induction with doxycycline for 6 h. (iii) For an additional control, the transcripts of parental, unmodified DG75 cells were analyzed prior to and after adding doxycycline for 6 h. All experiments were performed as triplicates. The analysis is based on Homo sapiens (human) genome assembly GRCh37 (hg19) from Genome Reference Consortium. Artificial ERCC Spike-in RNAs (Thermo Scientific) were added as external controls.

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Project description:ChIP-seq experiments with the antibody BZ1 directed against the EBV protein BZLF1 prior to and 15 hours post induction with doxycycline (100 ng/ml). The doxycycline regulated conditional BZLF1 allele is encoded by an inducible oriP plasmid vector stably introduced into DG75 cells. Experiments were performed as duplicates.

2021-02-07 | E-MTAB-8422 | biostudies-arrayexpress

ATAC-seq experiment in two versions of Raji cells with inducible BZLF1 variants prior to and after induction of EBV's lytic cycle by doxycycline.

Project description:The aim of the experiment was to identify accessible (open) chromatin regions in the genome prior to and post induction of EBV's lytic phase by the BZLF1 protein. Two versions of BZLF1 were employed: full-length and activation-domain (AD)-truncated BZLF1 non-induced and induced for 15 h. The experiments were performed as triplicates.

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Identification of chromatin interactions of 49 selected cellular loci prior to and post induction of BZFL1 in DG75 cells using the Capture-C technology.

Project description:Identification of specific chromatin interactions of 49 selected genes with the Capture-C technique in DG75 iBZLF1 cells prior to and 15 h after expression of EBV's protein BZLF1. The experiments were performed as triplicates.

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ChIP-seq against BZLF1 in DG75 cells (EBV-negative, human Burkitt lymphoma cell line)

Project description:The parental DG75 cell line is EBV negative and cannot express the viral BZLF1 protein, thus, this set of experiments served as a negative control. After ChIP, peaks were identified in both the human and viral (EBV (Human Herpesvirus 4) genome KF717093.1) genome. Only few peaks were identified. Experiments were performed as duplicates.

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Project description:RNA-seq data from DG75 cells with inducible BZLF1 prior to and after induction of EBV's lytic cycle by doxycycline.

| PRJEB34860 | ENA

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Project description:ChIP-seq against BZLF1 in DG75 cells (EBV-negative, human Burkitt lymphoma cell line)

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ATAC-seq experiment in two versions of Raji cells with inducible BZLF1 variants prior to and after induction of EBV's lytic cycle by doxycycline.

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