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Aquatic environmental monitoring using integrated Omics approaches

ABSTRACT: Quantitative transcriptomics in evaluating ovarian developmental effects in Atlantic cod (Gadus morhua) caged at a capped waste disposal site

PROVIDER: PRJEB34872 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	ERR3697640_1.fastq.gz	Fastqsanger.gz
	ERR3697640_2.fastq.gz	Fastqsanger.gz
	ERR3697650_1.fastq.gz	Fastqsanger.gz
	ERR3697650_2.fastq.gz	Fastqsanger.gz
	ERR3697641_1.fastq.gz	Fastqsanger.gz

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Project description:Gadus morhua (Atlantic cod)

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The study of Atlantic cod (Gadus morhua) spleen global gene expression response to intraperitoneal injection with formalin-killed atypical Aeromonas salmonicida.

Project description:This study was performed to validate the newly developed CGP Atlantic cod 20K oligonucleotide microarray. Atlantic cod (Gadus morhua) received an intraperitoneal injection of either formalin-killed, atypical Aeromonas salmonicida (Asal) or PBS and transcriptional profiles of spleen tissues from Asal-injected fish were compared to those from pre-injection control fish and PBS-injected control fish. Gene expression profiles resulting from this study were compared to those from suppression subtractive hybridization library studies, that were previously performed on the same samples, and to literature. Gene expression patterns of single genes were confirmed by QPCR analysis. This study has shown that the newly developed CGP Atlantic cod 20K oligo microarray platform is a valuable tool for cod genomic research.

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