Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Color Assay Tracing Repair

ABSTRACT: A scalable CRISPR/Cas9-based fluorescent reporter assay to study DNA double-strand break repair choice

PROVIDER: PRJEB35246 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	ERR4179520_1.fastq.gz	Fastqsanger.gz
	ERR4179520_2.fastq.gz	Fastqsanger.gz
	ERR4179521_1.fastq.gz	Fastqsanger.gz
	ERR4179521_2.fastq.gz	Fastqsanger.gz
	ERR4179522_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 18

Similar Datasets

TRIP assay of mismatch repair during single-strand annealing

Project description:Biases of DNA repair can shape the nucleotide landscape of genomes at evolutionary timescales. However, such biases have not yet been measured in chromatin for lack of technologies. Here we develop a genome-wide assay whereby the same DNA lesion is repaired in different chromatin contexts. We insert thousands of barcoded transposons carrying a reporter of DNA mismatch repair in the genome of mouse embryonic stem cells. Upon inducing a double-strand break between tandem repeats, a mismatch is generated when the single strand annealing repair pathway is used. Surprisingly, the mismatch repair machinery favors the same strand 60-80% of the time. The location of the lesion in the genome and the type of mismatch have little influence on the repair bias in this context.

2020-05-14 | GSE141211 | GEO

Saccharomyces cerevisiae: DSB mapping by Break-chip in a two-color experiment (G1 control vs. S phase samples)

2015-01-12 | GSE64446 | GEO

A fluorescent assay for cryptic transcription in Saccharomyces cerevisiae

Project description:A fluorescent assay for cryptic transcription in Saccharomyces cerevisiae

| PRJNA1049842 | ENA

The Dystonia Gene THAP1 Controls DNA Double Strand Break Repair Choice

Project description:The Dystonia Gene THAP1 Controls DNA Double Strand Break Repair Choice

| PRJNA647336 | ENA

Saccharomyces cerevisiae: DSB mapping by Break-chip in a two-color experiment (G1 control vs. S phase samples)

Project description:Break-chip (microarray-based double strand break mapping) analysis of mec1 cells recovering from 200 mM hydroxyurea in the presence or absence of 0.8 micromolar bathophenanthroline sulfonate (BPS). We asked if the presence of an iron chelator, BPS, during cell recovery from transient exposure to 200 mM hydroxyurea changes the global patterns of DNA double strand breaks (DSBs). We used a yeast checkpoint mutant, mec1, which has been shown to produce DSBs at replication forks after hydroxyurea was removed from the cell culture. We synchronously released cells from the G1/S transition into S phase in the presence of 200 mM hydroxyurea. After 1h treatment, the drug was removed and cells were allowed to recover in fresh medium for 1h in the presence or absence of 0.8 micromolar BPS. Recover (R) samples, R-1h-BPS and R-1h+BPS, as well as the G1 control samples were collected. Break-chip analysis was performed as previously described (Feng et al., G3(Bethesda) 2011 Oct;1(5):327-35. doi: 10.1534/g3.111.000554).

2015-01-12 | E-GEOD-64446 | biostudies-arrayexpress

ASF1 acts with RIF1 to promote DNA end-joining in BRCA1 deficient cells

Project description:RIF1 acts downstream of 53BP1 to coordinate DNA double strand break repair pathway choice between non-homologous end joining (NHEJ) and homologous recombination (HR). Here we identified ASF1 as an endogenous RIF1-associated protein. We showed that ASF1 forms complex with RIF1 and regulates RIF1-dependent functions in DNA damage response.

2022-06-09 | PXD027727 | Pride

Massively parallel splicing reporter assay on designed sequence libraries

Project description:Massively parallel splicing reporter assay on designed sequence libraries

| PRJNA545827 | ENA

Massively parallel reporter assay for identification of RNA switches

Project description:Massively parallel reporter assay for identification of RNA switches

| PRJNA1105614 | ENA

A fluorescent assay for cryptic transcription in Saccharomyces cerevisiae [MNase-seq]

Project description:A fluorescent assay for cryptic transcription in Saccharomyces cerevisiae [MNase-seq]

| PRJNA1049345 | ENA

A fluorescent assay for cryptic transcription in Saccharomyces cerevisiae [RNA-seq]

Project description:A fluorescent assay for cryptic transcription in Saccharomyces cerevisiae [RNA-seq]