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Soil and Lactuca sativa resistome under exposure of antibiotics thorugh irrigation water.

ABSTRACT: In this study we assessed the effects of indirect exposure of antibiotics via irrigation water in microbiomes and resistomes composition of soil and Lactuca Sativa (Lettuce) in a controlled setting. We collected samples from soil and roots, and microbiome composition of all samples went through shotgun-sequencing.

PROVIDER: PRJEB35612 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR3697022_1.fastq.gz	Fastqsanger.gz
	ERR3697022_2.fastq.gz	Fastqsanger.gz
	ERR3697023_1.fastq.gz	Fastqsanger.gz
	ERR3697023_2.fastq.gz	Fastqsanger.gz
	ERR3697024_1.fastq.gz	Fastqsanger.gz

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Soil and Root's Microbiome and Resistome antibiotic exposure thougr irrigation water

Project description:In this study we assessed the effects of indirect exposure of antibiotics via irrigation water in microbiomes and resistomes composition of soil and Lactuca Sativa (Lettuce) in a controlled setting. We collected samples from soil and roots, and microbiome composition of all samples were analyzed by 16s rDNA amplicon sequencing

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Interactive effects of depth and differential irrigation on soil microbiome composition and functioning_PNNLSoilsSFA_Naylor

Project description:GCMS datasets for the soil depth manuscript Abstract Two factors that are well-known to influence soil microbiomes include the depth of the soil as well as the level of moisture. Previous works have demonstrated that climate change will increase the incidence of drought in soils, but it is unknown how fluctuations in moisture availability affect soil microbiome composition and functioning down the depth profile. Here, we investigated soil and wheatgrass rhizosphere microbiomes in a common field setting under four different irrigation regimes and three depths. We demonstrated that there is a significant interactive effect, where fluctuations in soil moisture more strongly influence soil microbiomes at the surface layer than in deeper layers, including for soil community composition, diversity, and for functional profiles. Meanwhile, in rhizosphere communities the influence of irrigation was similar across the different depths, although there were slight discrepancies between the two cultivars of wheatgrass used. The lessened response of deeper soil microbiomes to changes in irrigation may be due to higher incidence of slow-growing, stress-resistant microbes.

2022-11-07 | MSV000090655 | MassIVE

Mexican maize landraces under drought stress and recovery irrigation

Project description:Transcriptional profiling of three mexican maize landraces under 10, and 17 days stress and recovery irrigation

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Mexican maize landraces under drought stress and recovery irrigation

Project description:Transcriptional profiling of three mexican maize landraces under 10, and 17 days stress and recovery irrigation A dye balanced modified loop design was implemented. Two biological replicates (pooling five representative plants) representing each sampling point for each genotype were obtained for purified RNA from 120 randomly chosen seedlings. This experiment involved a total of forty-eight (24 sets) of microarray hybridizations, including direct and dye swap comparisons between treatments as well as across the three landraces. This design allowed us to determine differences in gene expression between the three different landraces under drought stress (10 and 17 days) and at recovery irrigation compared to irrigated controls.

2010-05-16 | E-GEOD-14728 | biostudies-arrayexpress

Grape berry developmental series comparing irrigation treatments in a vineyard in Wingara, Victoria, Australia (WIN-05)

Project description:Changes in gene expression during berry development during a grape growing season were analysed. The effect on gene expression of different viticultural practises during grape berry development was investigated in this study by comparing two irrigation methods (standard versus prolonged deficit irrigation). Grape berries were collected and pooled on a weekly basis to obtaining a developmental series comprising of 17 developmental stages from flowering until harvest across the grape growing season for both standard and prolonged deficit irrigated vines. Gene expression patterns during development and between pruning treatments were obtained. Keywords: Time course, developmental series and treatments

2018-11-22 | GSE7679 | GEO

GNPS - Interactive effects of depth and differential irrigation on soil microbiome composition and functioning_PNNLSoilsSFA_Naylor

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Irrigation Water and Fecal 16S rRNA

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Molecular mechanism of negative pressure irrigation inhibiting root growth and improving water use efficiency in maize

Project description:NPI enriched more down-regulation genes involved in starch and sucrose metabolism, phenylpropanoid biosynthesis, amino sugar and nucleotide sugar metabolism, glycolysis/gluconeogenesis pathway, leading to restricted root growth. Three GO terms including channel activity, water transport, and water channel activity caused a high degree of gene enrichment. Thirteen genes related to water absorption and transport such as ZM00001D003006, ZM00001D014285 et al. were highly up-regulated, which might be the key genes for the significant increase in water use efficiency of maize under negative pressure irrigation.

2021-07-22 | GSE180352 | GEO

Potato Irrigation Experiment

Project description:It was verified the impact of irrigation treatments (wet, optimal, dry and very dry) and three potato cultivars on soil microbial community.

| PRJEB27037 | ENA

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