Project description:The presence of DNA methylation in beetles (Coleoptera) has only been investigated with bisulfite sequencing of Tribolium castaneum, which produced no evidence of DNA methylation. Here, we used whole genome bisulfite sequencing to assay if DNA methylation was present in another beetle, Nicrophorus vespilloides. We used T. castaneum as a negative control.
Project description:This series examines gene expression in the anterior midgut at several time points (2, 4, 8, & 16 h) after topical application of juvenile hormone III (JHIII) or acetone (control) to adult beetles of both sexes. Keywords = North American pine engraver beetle Keywords = anterior midgut Keywords = juvenile hormone Keywords = pheromone biosynthesis Keywords = Coleoptera Keywords = Scolytidae Keywords: other
Project description:This series examines gene expression in the anterior midgut at several time points (2, 4, 8, & 16 h for males, 8 h for females) after topical application of juvenile hormone III (JHIII) or acetone (control) to adult beetles. In addition, gene expression in male anterior midguts were examined 24 h after phloem feeding or in unfed beetles. Keywords: North American pine engraver beetle; anterior midgut; juvenile hormone; pheromone biosynthesis; Coleoptera; Scolytidae Publication reference: Reference Type: Book Section Authors: Tittiger, Claus; Keeling, Christopher I.; Blomquist, Gary J. Year: 2005 Title: Some insights into the remarkable metabolism of the bark beetle midgut. Editor: Romeo, J.T. Book Title: Chemical ecology and phytochemistry of forest ecosystems City: Toronto Publisher: Elsevier Volume: 39 Pages: 57-78 Series Title: Recent Advances in Phytochemistry Keywords: other