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Single-nucleus transcriptomics reveals functional compartmentalization in syncytial skeletal muscle cells

ABSTRACT: Single-nucleus transcriptomics reveals functional compartmentalization in syncytial skeletal muscle cells

PROVIDER: PRJEB35920 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR3773420_1.fastq.gz	Fastqsanger.gz
	ERR3773420_2.fastq.gz	Fastqsanger.gz
	ERR3773430_1.fastq.gz	Fastqsanger.gz
	ERR3773430_2.fastq.gz	Fastqsanger.gz
	ERR3773440_1.fastq.gz	Fastqsanger.gz

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Single-nucleus transcriptomics reveals functional compartmentalization in syncytial skeletal muscle cells

Project description:Syncytial skeletal muscle cells contain hundreds of nuclei in a shared cytoplasm. Using single nucleus RNA-sequencing (snRNAseq) of isolated nuclei from muscle fibers, we investigated nuclear heterogeneity and transcriptional dynamics in uninjured and regenerating muscle.

2020-10-07 | E-MTAB-8623 | biostudies-arrayexpress

Single-nucleus transcriptomics reveals functional compartmentalization in syncytial skeletal muscle cells.

Project description:Syncytial skeletal muscle cells contain hundreds of nuclei in a shared cytoplasm. We investigated nuclear heterogeneity and transcriptional dynamics in the uninjured and regenerating muscle using single-nucleus RNA-sequencing (snRNAseq) of isolated nuclei from muscle fibers. This revealed distinct nuclear subtypes unrelated to fiber type diversity, previously unknown subtypes as well as the expected ones at the neuromuscular and myotendinous junctions. In fibers of the Mdx dystrophy mouse model, distinct subtypes emerged, among them nuclei expressing a repair signature that were also abundant in the muscle of dystrophy patients, and a nuclear population associated with necrotic fibers. Finally, modifications of our approach revealed the compartmentalization in the rare and specialized muscle spindle. Our data identifies nuclear compartments of the myofiber and defines a molecular roadmap for their functional analyses; the data can be freely explored on the MyoExplorer server ( https://shiny.mdc-berlin.de/MyoExplorer/ ).

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Skeletal Muscle Transcriptomics

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Single-nucleus RNA-seq identifies transcriptional heterogeneity in multinucleated skeletal myofibers

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