Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Pseudomonas aeruginosa biofilms response to alginate therapy

ABSTRACT: Phenotypic and genotypic adaptations in Pseudomonas aeruginosa biofilms following long-term exposure to an alginate oligomer therapy

PROVIDER: PRJEB36146 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other
	ERR4057800_1.fastq.gz	Fastqsanger.gz
	ERR4057800_2.fastq.gz	Fastqsanger.gz
	ERR4058310_1.fastq.gz	Fastqsanger.gz
	ERR4058310_2.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 83

Similar Datasets

The architecture and pathogenesis of Pseudomonas-Candida biofilms in infections and their influence on the microenvironment

Project description:Interkingdom Pseudomonas-Candida biofilms is a human pathogen associated with acute and chronic infection. This study examine hoe their structural organization influences their in vivo microenvironment, which in turns affects the interaction of Pseudomonas-Candida biofilms with host immune responses. NCI-H292 grown P. aeruginosa biofilms with and without C. albicans biofilms were used for proteomic analysis. Biofilms were demonstrated in all groups which have 357 proteins from proteomic analysis including i) proteins in PA+CA were higher than PA (76 proteins, such as sigma factor AlgU negative regulatory protein; MucA) and ii) proteins in PA+CA were lower than PA (281 proteins, including alginate genes such as Alginate biosynthesis transcriptional regulatory protein; AlgB). Although most of Pseudomonas proteins in PA+CA were lower than PA due to the lower initial bacteria in preparation of mixed-organism biofilms, mucA overexpressed in PA+CA indicating that high alginate production that transcriptionally controlled by algU-mucABCD genes. These results demonstrated that Pseudomonas-Candida biofilms produced alginate more compared to Pseudomonas biofilms on lungs.

2022-10-13 | PXD034528 | Pride

Identification of adsorbing human plasma proteins on alginate gel beads

Project description:The biocompatibility of immunoisolation devices is a major challenge for their use in cellular therapies. Utilisation of alginate for the encapsulation of pancreatic islets as a potential cellular therapy for type 1 diabetes has shown to be limited in terms of long-term graft survival, due to pericapsular fibrotic overgrowth. Fibrotic overgrowth is a complex process that involves several factors, including protein adsorption. This work investigates the protein adsorption profiles of plasma incubated alginate beads for the identification of key proteins that may play a role in the biocompatibility of this biomaterial.

2023-03-10 | PXD009135 | Pride

Dynamic study of In vitro murine follicle maturation in 3% alginate gels

Project description:Multilayer murine follicles were grown in 3% alginate and follicles were isolated for transcriptomics analysis at day 0, 2, 4,5,6,and 8 after encapsulation. This data complements to the GSE42795, where follicles were grown in vitro in 0.5% alginate gels

2014-06-01 | GSE55969 | GEO

alginate converting by MCF

Project description:alginate converting by MCF Metagenome

| PRJNA509496 | ENA

Glutaraldehyde resistance mechanisms in Pseudomonas fluorescens and Pseudomonas aeruginosa biofilms

Project description:The objective of the current study was to understand the glutaraldehyde resistance mechanisms in P. fluorescens and P. aeruginosa biofilms. Glutaraldehyde is a common biocide used in various industries to control the microbial growth. Recent reports of emergence of glutaraldehyde resistance in several bacterial species motivated this study to understand the genetic factors responsible got glutaraldehyde resistance. Using a combination of phenotypic assays, chemical genetic assays and RNA-seq, we demonstrate that novel efflux pump, polyamine biosynthesis, lipid biosynthesis and phosphonate degradation play significant role in glutaraldehyde resistance and post-glutaraldehyde recovery of Psudomonad biofilms.

2015-04-06 | GSE64448 | GEO

Gene expression in cells encapsulated within different types of alginate microcapsules

Project description:D1 multipotent mesenchymal stromal cells (D1‐MSCs,ATCC® CRL 12424TM) and genetically modified with the lentiviral vector pSIN‐EF2‐Epo‐Pur to express erythropoietin (EPO). D1‐MSCs genetically modified to release EPO were immobilized into 3D alginatepoly‐ L‐lysine‐alginate (APA) microcapsules. Since different formulations of alginate 1.5%, poly‐L‐lysine 0.05%, alginate 0.1% and washings were designed, two types of APA microcapsules were obtained: Biological microcapsules (made of Biological formulations) and Technological microcapsules (made of Technological formulations). The expression of the cells under different conditions of these two types of microcapsules have been analyzed. The present analysis proved that the mechanical properties of the matrix in which cells are enclosed influences drastically gene expression

2017-10-30 | E-MTAB-5786 | biostudies-arrayexpress

Dynamic study of In vitro murine follicle maturation in 3% alginate gels

2014-06-01 | E-GEOD-55969 | biostudies-arrayexpress

Alginate encapsulation of differentiating hiPSCs

Project description:Here we characterize the impact of cell confinement on the pancreatic islet signature during the guided differentiation of alginate encapsulated human induced pluripotent stem cells.

2020-02-12 | PXD012704 | Pride

Biofilms

Project description:Biofilms Raw sequence reads

| PRJNA1034779 | ENA

DeMMO Biofilms

Project description:DeMMO Biofilms

| PRJNA602862 | ENA