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Identifying crossover locations in Arabidopsis thaliana wild type and asy1 Col-0 × Ws-4 F2 populations using genotyping-by-sequencing

ABSTRACT: Identifying crossover locations in Arabidopsis thaliana wild type and asy1 Col-0 × Ws-4 F2 populations using genotyping-by-sequencing

PROVIDER: PRJEB36377 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	ERR3835540_1.fastq.gz	Fastqsanger.gz
	ERR3835540_2.fastq.gz	Fastqsanger.gz
	ERR3835550_1.fastq.gz	Fastqsanger.gz
	ERR3835550_2.fastq.gz	Fastqsanger.gz
	ERR3835560_1.fastq.gz	Fastqsanger.gz

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Identifying crossover locations in Arabidopsis thaliana wild type and asy1 Col-0 × Ws-4 F2 populations using genotyping-by-sequencing

Project description:Genomic DNA from 187 wild type and 169 asy1 Col-0 x Ws-4 F2 individuals was extracted using CTAB and used to generate sequencing libraries as described (Lawrence et al, 2019 Current Biology). Sequencing data was analysed to identify crossovers using the TIGER pipeline as previously described (Rowan et al, 2015 G3 (Bethesda); Yelina et al, 2015 Genes & Dev; Lawrence et al, 2019 Current Biology).

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Project description:Genomic DNA from 192 Col x Ler F2 individuals was extracted using CTAB and used to generate sequencing libraries as described (Rowan et al; Yelina et al), with the following modifications. DNA was extracted from 3 rosette leaves of 5 week old plants and 150 ng of DNA used as input for each library. DNA shearing was carried out for 20 minutes at 37C with 0.4U of DNA Shearase (Zymo research). The barcoded adapters used for library construction are listed in Rowan et al. Each set of 96 libraries was sequenced on one lane of an Illumina NextSeq500 instrument (300-cycle Mid Output run). Sequencing data was analysed to identify crossovers as previously reported, using the TIGER pipeline (Rowan et al; Yelina et al). Rowan BA, Patel V, Weigel D, Schneeberger K. Rapid and Inexpensive Whole-Genome Genotyping-by-Sequencing for Crossover Localization and Fine-Scale Genetic Mapping. G3 (Bethesda). 2015;5: 38598. Yelina NE, Lambing C, Hardcastle TJ, Zhao X, Santos B, Henderson IR. DNA methylation epigenetically silences crossover hot spots and controls chromosomal domains of meiotic recombination in Arabidopsis. Genes Dev. 2015;29: 2183202.

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Identifying crossover locations in an Arabidopsis thaliana HEI10 Col x Ler F2 population using genotyping by sequencing.

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