Project description:RNA-sequencing was performed to investigate the role of the Candida albicans protein kinase Sky2 in metabolic adaptation to alternative carbon sources. The Candida albicans wildtype strain SC5314 and a sky2 deletion mutant were grown in medium with either glucose (3h), malic acid (6h) or succinic acid (6h) as the sole carbon source. Because the sky2 deletion mutant showed decreased growth in media that contain either malic acid or succinic acid as the sole carbon source, we wanted to investigate the transcriptional changes in the sky2 mutant grown on these carbon sources.
Project description:This study evaluated glycine as a sole carbon source in an EBPR sequencing batch reactor, demonstrating effective phosphorus removal comparable to systems fed with mixed substrates. Microbial and genome-resolved analyses revealed a community with complementary metabolic roles, highlighting its contribution to phosphorus removal dynamics.
Project description:Cross-feeding is fundamental to the diversity and function of microbial communities. However, identification of cross-fed metabolites is often challenging due to the universality of metabolic and biosynthetic intermediates. Here, we use 13C isotope tracing in peptides to elucidate cross-fed metabolites in cocultures of Saccharomyces cerevisiae and Lactococcus lactis. The community was grown on lactose as the main carbon source with either glucose or galactose fraction of the molecule labelled with 13C. Data analysis allowing for the possible mass-shifts yielded hundreds of peptides for which we could assign both species identity and labelling degree. The labelling pattern showed that the yeast utilized galactose and, to a lesser extent, lactic acid shared by L. lactis as carbon sources. While the yeast provided essential amino acids to the bacterium as expected, the data also uncovered a complex pattern of amino acid exchange. The identity of the cross-fed metabolites was further supported by metabolite labelling in the co-culture supernatant, and by diminished fitness of a galactose-negative yeast mutant in the community. Together, our results demonstrate the utility of 13C-based proteomics for uncovering microbial interactions.
