Project description:Body-fluid specific marker provide important information for crime scene, but some marker such as mRNA and protein can provide wrong information because cross-reaction. We used microarrays to identify body-fluid specific miRNA marker for forensic use.
Project description:Body-fluid specific marker provide important information for crime scene, but some marker such as mRNA and protein can provide wrong information because cross-reaction. We used microarrays to identify body-fluid specific miRNA marker for forensic use. Genome-wide miRNA profiling in 4 type of body fluid to identify each body fluid specific marker
Project description:tRNAs are heavily decorated with post-transcriptional modifications (tRNA modifications). Profile of tRNA modifications in non-model organisms are largely uncharacterized. Here using high-throughput sequencing, sites and frequency of tRNA modifications are predicted in Vibrio cholerae and Escherichia coli. During cDNA synthesis, some modifications cause misincorporation of a wrong base or termination of reverse transcription (RT). Using these RT-derived signatures we aim to explore organism-specific modifications and to track changes in modification frequency among different cellular conditions.
