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Gene expression analysis in leaves of sugarcane genotypes contrasting in biomass production

ABSTRACT: Gene expression analysis in leaves of sugarcane genotypes contrasting in biomass production

PROVIDER: PRJEB38368 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR4165190_1.fastq.gz	Fastqsanger.gz
	ERR4165190_2.fastq.gz	Fastqsanger.gz
	ERR4165200_1.fastq.gz	Fastqsanger.gz
	ERR4165200_2.fastq.gz	Fastqsanger.gz
	ERR4165210_1.fastq.gz	Fastqsanger.gz

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Leaf transcriptome profiling of contrasting sugarcane genotypes for drought tolerance under field conditions

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Control of sucrose accumulation in sugarcane (Saccharum spp. hybrids) involves miRNA-mediated regulation of genes and transcription factors associated with sugar metabolism

Project description:Sugarcane established industrial crop providing sugar, ethanol and biomass-derived electricity around the world. Cane sugar content is an important, breeding target, but its improvement remains very slow in many breeding programmes. Biotechnology strategies to improve sucrose accumulation made little progress at crop level, mainly due to the limited understanding of its regulation. MiRNAs regulate many metabolic processes in plants. However, their roles and target genes associated with sugarcane sucrose accumulation remains unknown. Here, we conducted high-throughput sequencing of transcriptome, small RNAs and degradome of leaves and stem of two sugarcane genotypes with contrasting sucrose content from the early to late stages of sucrose accumulation stages, which provided more insights into miRNA-associated gene regulation during sucrose accumulation. Transcriptome analysis identified 18,722 differentially expressed genes (DEGs) between both genotypes during sucrose accumulation. The major DEGs identified were involved in starch and sucrose metabolism, and photosynthesis etc. miRNA sequencing identified 563 known and 281 novel miRNAs from both genotypes during sucrose accumulation. Of these, 311 miRNAs were differentially expressed.752 targets of 368 miRNAs (609 targets for 260 known miRNAs and 168 targets for 108 novel miRNAs) were identified by degradom sequencing.Several known and novel miRNAs and their target genes associated with sugar metabolism, sugar transport and sucrose storage were identified in this study.This new insight into the complex network of sucrose accumulation in sugarcane will help identify candidate targets for sucrose improvement in sugarcane through molecular means.

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