Project description:Transcritome study of C.elegans exposed to multiple, different bacterial pathogens. Experiments were performed in set-replicates of either 3 or 5.<br> There are 3 for samples: Aeromonas hydrophila, Enterococcus faecalis, Erwinia carotovora and Photorhabdus luminescens. <br> There are 5 for samples: Serratia marcesens and Escherichia coli (control).<br>
Project description:An integrated approach was used to identify genes associated with resistance to Aeromonas hydrophila, an important bacterial pathogen causing aeromoniasis in rohu Labeo rohita. DNA polymorphism and gene expression profiling in lines of L. rohita selected for resistance or susceptibility to challenge with A. hydrophila, and grown in a common environment, were studied using Illumina mRNA-seq of selectively pooled RNA samples.
Project description:This project was designed to observe changes in proteins expression and toxicity proteins expression of Aeromonas hydrophila under normal and iron restriction conditions.
Project description:An integrated approach was used to identify genes associated with resistance to Aeromonas hydrophila, an important bacterial pathogen causing aeromoniasis in rohu Labeo rohita. DNA polymorphism and gene expression profiling in lines of L. rohita selected for resistance or susceptibility to challenge with A. hydrophila, and grown in a common environment, were studied using Illumina mRNA-seq of selectively pooled RNA samples. mRNA-seq of pooled samples from resistant and susceptible lines of rohu for expression characterisation and SNP detection
Project description:Fish skin is a critical regulatory organ, serving not only as a physical barrier to pathogen entry, but also as a sophisticated integrator of aquatic environmental, social and nutritional cues through roles in immunity, osmoregulation, and endocrine signaling. Integral to the complexity of teleost skin is the mucus layer secreted by epidermal goblet cells. Pathogen invasion can disrupt this delicate homeostasis with profound impacts on signaling throughout the organism. Here, we investigated the transcriptional effects of virulent A. hydrophila infection in blue catfish skin, Ictalurus furcatus. We utilized an 8X60K Agilent microarray to examine gene expression profiles at critical early timepoints following challenge—2 h, 12 h, and 24 h. Expression of a total of 1,155 unique genes was significantly perturbed during at least one timepoint. We observed dysregulation of a number of genes involved in including antioxidant/apoptosis, cytoskeletal rearrangement, immune response, junctional/adhesion, and proteases. In particular, A. hydrophila infection rapidly altered a number potentially critical lectins, chemokines, interleukins, and other mucosal factors in a manner predicted to enhance its ability to adhere and invade the catfish host. Two-condition experiment, control vs. infected skin. Biological replicates: 3 control replicates, 3 infected replicates.3 timepoints
Project description:This study investigates the immune responses in the stomach of channel catfish (Ictalurus punctatus) under different feeding statuses and physical stress (fin-clipping) after infection with Aeromonas hydrophila. Transcriptomic analysis revealed early activation of Toll-like receptor (TLR) signaling and pro-inflammatory pathways in the fed groups, which led to significant tissue damage as observed in histopathological evaluations. Unfed groups showed delayed immune responses with less tissue damage but higher levels of apoptosis, particularly in the fin-clipped group. Histopathological examination at 2 hours post-challenge (HPC) revealed minimal to moderate edema in the lamina propria and submucosa across all treatment groups. By 4 HPC, mild to moderate edema was noted, accompanied by lymphoplasmacytic and granulocytic infiltration. At 8 HPC, multifocal hemorrhage and erosions in the superficial epithelium, along with moderate to severe transmural edema, were observed. These histological findings, particularly in the fed fin-clipped group, reflect the correlation between immune overactivation and tissue damage leading to higher mortality.