Project description:Transcritome study of C.elegans exposed to multiple, different bacterial pathogens. Experiments were performed in set-replicates of either 3 or 5.<br> There are 3 for samples: Aeromonas hydrophila, Enterococcus faecalis, Erwinia carotovora and Photorhabdus luminescens. <br> There are 5 for samples: Serratia marcesens and Escherichia coli (control).<br>
Project description:An integrated approach was used to identify genes associated with resistance to Aeromonas hydrophila, an important bacterial pathogen causing aeromoniasis in rohu Labeo rohita. DNA polymorphism and gene expression profiling in lines of L. rohita selected for resistance or susceptibility to challenge with A. hydrophila, and grown in a common environment, were studied using Illumina mRNA-seq of selectively pooled RNA samples.
Project description:This project was designed to observe changes in proteins expression and toxicity proteins expression of Aeromonas hydrophila under normal and iron restriction conditions.
Project description:An integrated approach was used to identify genes associated with resistance to Aeromonas hydrophila, an important bacterial pathogen causing aeromoniasis in rohu Labeo rohita. DNA polymorphism and gene expression profiling in lines of L. rohita selected for resistance or susceptibility to challenge with A. hydrophila, and grown in a common environment, were studied using Illumina mRNA-seq of selectively pooled RNA samples. mRNA-seq of pooled samples from resistant and susceptible lines of rohu for expression characterisation and SNP detection
Project description:Fish skin is a critical regulatory organ, serving not only as a physical barrier to pathogen entry, but also as a sophisticated integrator of aquatic environmental, social and nutritional cues through roles in immunity, osmoregulation, and endocrine signaling. Integral to the complexity of teleost skin is the mucus layer secreted by epidermal goblet cells. Pathogen invasion can disrupt this delicate homeostasis with profound impacts on signaling throughout the organism. Here, we investigated the transcriptional effects of virulent A. hydrophila infection in blue catfish skin, Ictalurus furcatus. We utilized an 8X60K Agilent microarray to examine gene expression profiles at critical early timepoints following challenge—2 h, 12 h, and 24 h. Expression of a total of 1,155 unique genes was significantly perturbed during at least one timepoint. We observed dysregulation of a number of genes involved in including antioxidant/apoptosis, cytoskeletal rearrangement, immune response, junctional/adhesion, and proteases. In particular, A. hydrophila infection rapidly altered a number potentially critical lectins, chemokines, interleukins, and other mucosal factors in a manner predicted to enhance its ability to adhere and invade the catfish host. Two-condition experiment, control vs. infected skin. Biological replicates: 3 control replicates, 3 infected replicates.3 timepoints
Project description:The study focuses on the intestinal immune responses of channel catfish (Ictalurus punctatus) under various feeding and fin-clipping conditions after infection with Aeromonas hydrophila. Transcriptomic data revealed significant activation of pathogen recognition pathways (TLR signaling and C-type lectins) and pro-inflammatory cytokines, particularly in the fed groups. Histopathological findings at 2 hours post-challenge (HPC) showed minimal to moderate expansion of the lamina propria by edema in the intestines of the treated groups. At 4 HPC, mild to moderate edema with lymphocytes and plasma cells was observed, indicating early immune cell infiltration. By 8 HPC, degenerative changes in the superficial epithelium and hemorrhage were evident, especially in the fed fin-clipped group, which correlates with increased mortality in this group. The unfed groups demonstrated delayed but sustained immune responses, marked by lower histological damage compared to the fed groups. These findings provide insights into how nutritional status and fin-clipping shape the intestinal immune response and tissue damage during infection.