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RNA-Seq of Arabidopsis thaliana plants primed by insect eggs from Piereis brassicae and triggered by insect herbivory against untreated controls in two different developmental phases (vegetative vs. reproductive)

ABSTRACT: RNA-Seq of Arabidopsis thaliana plants primed by insect eggs from Piereis brassicae and triggered by insect herbivory against untreated controls in two different developmental phases (vegetative vs. reproductive)

PROVIDER: PRJEB38761 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR4221380_1.fastq.gz	Fastqsanger.gz
	ERR4221380_2.fastq.gz	Fastqsanger.gz
	ERR4221390_1.fastq.gz	Fastqsanger.gz
	ERR4221390_2.fastq.gz	Fastqsanger.gz
	ERR4221400_1.fastq.gz	Fastqsanger.gz

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RNA-Seq of Arabidopsis thaliana plants primed by insect eggs from Pieris brassicae and triggered by insect herbivory against untreated controls in two different developmental phases (vegetative vs. reproductive)

Project description:Plants can cope with stress better if they experience a mild form of the stress before the actual \\"real\\" stress event. In Arabidopsis thaliana it is known that plants that harboured eggs of the White cabbage butterfly (Pieris brassicae) before larval feeding can defend better against the herbivore stress. The main aim of the experiment was to compare the priming effect induced by insect egg deposition of Pieris brassicae between vegetative and reproductive (first open flowers) Arabidopsis thaliana plants on the transcriptional level. We used a full factorial setup consisting of a) untreated control plants , b) plants which experienced eggs for 6 days without larval feeding after that period, c) plants which experienced no eggs before larval feeding for 24 hours d) plants which experienced eggs for 6 days and larval herbivory for 24 hours. This setup was conducted with 6 week old vegetative plants and 10 week old reproductive plants were the first flowers were open. For all treatments leaf tissue from the leaves that experienced egg oviposition and/or larval feeding were collected. From reproductive plants flower buds were collected as well.

2022-05-10 | E-MTAB-9196 | biostudies-arrayexpress

RNA-Seq of Arabidopsis thaliana and Arabidopsis lyrata plants primed by insect eggs from Pieris brassicae and triggered by insect herbivory

Project description:In Arabidopsis thaliana it is known that plants that harboured eggs of the White cabbage butterfly (Pieris brassicae) before larval feeding can defend better against the herbivore stress. As Arabidopsis thaliana is an annual plant we were interested in the plant defense and possible priming reaction of the closest biannual/perennial relative Arabidopsis lyrata. The main aim of the experiment was to compare the effect induced by insect egg deposition of Pieris brassicae between A. thaliana and A. lyrata plants grown in parallel on the transcriptional level. We used a full factorial setup consisting of a) untreated control plants , b) plants which experienced eggs for 6 days without larval feeding after that period, c) plants which experienced no eggs before larval feeding for 24 hours d) plants which experienced eggs for 6 days and larval herbivory for 24 hours. This setup was conducted with 7 week old vegetative plants For all treatments leaf tissue from the leaves that experienced egg oviposition and/or larval feeding were collected.

2024-12-18 | E-MTAB-12653 | biostudies-arrayexpress

Gene expression in Arabidopsis leaves after P. brassicae egg deposition and/or herbivory by P. brassicae larvae

Project description:Plants can prepare their defense of impending herbivory, when they previously perceived eggs deposited by herbivores insect which reliably indicates that larvae will soon hatch and feed on the plants. Using Arabidopsis thaliana and Pieris brassicae we addressed the question whether prior egg deposition affects transcriptional changes in feeding-damaged plants. We determined the transcriptomes of Arabidopsis leaves that (i) were for six days deposited with eggs and suffered larval feeding for two days, (ii) suffered larval feeding for two days without prior egg deposition or (iii) were left untreated.

2018-10-04 | GSE94446 | GEO

Gene expression in Arabidopsis leaves after P. brassicae egg deposition, chilling, and/or herbivory by P. brassicae larvae

2016-06-23 | GSE69623 | GEO

Gene expression in Arabidopsis leaves after P. brassicae egg deposition, chilling, and/or herbivory by P. brassicae larvae

Project description:Plant defence against insects is well known to be affected by previous exposure to cues warning of herbivory. Using Arabidopsis thaliana and the herbivore Pieris brassicae, we addressed the question whether the maintenance of the effects of the warning cue depends on its reliability. We determined the transcriptomes of Arabidopsis leaves that were treated by P. brassicae egg deposition (i) five days after oviposition, (ii) one day after removal of the eggs following the egg treatment, (iii) three days after removal of the eggs, (iv) after two days of herbivory that started one day after removal of the eggs; or that were treated by chilling (v) five days after transfer to 4Â°C, (vi) one day after transfering the plants to 20Â°C following the chilling treatment, (vii) three days after transfering the plants to 20Â°C, (viii) after two days of herbivory that started one day after transfering the plants to 20Â°C. Arabidopsis thaliana Col-0 wild type plants were grown under short day conditions (10 h/14 h light/dark) at 20Â°C for 7 weeks. Subsequently (i) the plants were transferred to 4Â°C for 5 days, or (ii) Pieris brassicae deposited ca. 40 eggs on leaf 17 where they remained for five days, or (iii) as controls plants grew untreated for five days. Next, the plants were transferred back to 20Â°C and the eggs were removed, respectively. Next, all plants rested for 1 day at 20Â°C. Next, P. brassicae larvae were allowed to feed for 2 days on leaf 17 adjacent to the former egg deposit site or at a respective leaf region of chilling-treated or untreated plants. Control plants were not exposed to larvae. From all treated and untreated plants material from a leaf region proximal to the egg deposition and/or feeding site was harvested for transcriptome analysis.

2016-06-23 | E-GEOD-69623 | biostudies-arrayexpress

Brassica nigra challenged by Pieris brassicae, eggs of Pieris brassicae, Xanthomonas campestris pv. raphani, Brevicoryne brassicae or by combined stresses eggs of P. brassicae / P. brassicae, X. campestris / P. brassicae, B. brassicae / P. brassicae

Project description:Brassica nigra plants, a Brassicaceae close to Arabidopsis thaliana, was used for combined stresses experiments. In this study, we performed a whole-genome microarray analysis on five-week-old plants and compared untreated plants and plants treated different single or dual stresses: the larvae Pieris brassicae, egg extract of Pieris brassicae, the bacterial Xanthomonas campestris pv. raphani, the aphid Brevicoryne brassicae or by combined stresses eggs of P. brassicae / P. brassicae, X. campestris / P. brassicae, B. brassicae / P. brassicae.

2017-03-31 | E-MTAB-5059 | biostudies-arrayexpress

RNA-Seq of Arabidopsis thaliana and Arabidopsis lyrata plants primed by insect eggs from Pieris brassicae and triggered by insect herbivory

Project description:RNA-Seq of Arabidopsis thaliana and Arabidopsis lyrata plants primed by insect eggs from Pieris brassicae and triggered by insect herbivory

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Project description:RNA-Seq of Arabidopsis thaliana plants primed by insect eggs from Pieris brassicae and triggered by insect herbivory against untreated controls in two different developmental phases (vegetative vs. reproductive)

2023-06-16 | E-MTAB-9196 | ExpressionAtlas

Solanum dulcamara's response to insect eggs comprises ovicidal hydrogen peroxide production

Project description:Plants can respond to insect oviposition but little is known about which responses directly target the insect eggs and how. Here, we reveal a mechanism by which the bittersweet nightshade, Solanum dulcamara, kills the eggs of a generalist noctuid herbivore. The plant responded at the site of oviposition by Spodoptera exigua with neoplasm and chlorotic tissue formation, accumulation of reactive oxygen species and induction of defence genes and proteins. Transcriptome analysis revealed that these responses were reflected in the transcriptional reprogramming of the egg-laden leaf. The plant-mediated egg mortality on S. dulcamara was not present on a genotype lacking chlorotic leaf tissue at the oviposition sites on which the eggs are exposed to less hydrogen peroxide. As exposure to hydrogen peroxide increased egg mortality, while catalase supplementation prevented the plants from killing the eggs, our results suggest that ROS formation directly acts as an ovicidal plant response of S. dulcamara.

2017-06-21 | GSE100221 | GEO

Gene expression in Arabidopsis leaves after P. brassicae egg deposition

Project description:Characterization of Arabidopsis thaliana`s transcriptional response to Pieris brassicae eggs

2018-10-04 | GSE114041 | GEO