Project description:TRAP translational profiling is a method that allows investigators to genetically characterize specific cell types in complex tissues such as mouse brain. Using this technique we obtained RNA-Seq data from actively translating transcripts present in endothelial cells in the cerebral cortex of adult Abcb1a-EGFP/Rpl10a (ES3026) mice.
Project description:TRAP translational profiling is a method that allows investigators to genetically characterize specific cell types in complex tissues such as mouse brain. Using this technique we obtained RNA-Seq data from actively translating transcripts present in pyramidal neurons in the cerebral cortex of adult Cbln1-EGFP/Rpl10a (NIDA030) mice.
Project description:TRAP translational profiling is a method that allows investigators to genetically characterize specific cell types in complex tissues such as mouse brain. Using this technique we obtained RNA-Seq data from actively translating transcripts present in pyramidal neurons in the cerebral cortex of adult Htr4-EGFP/Rpl10a (ES1299) mice.
Project description:TRAP translational profiling is a method that allows investigators to genetically characterize specific cell types in complex tissues such as mouse brain. Using this technique we obtained RNA-Seq data from actively translating transcripts present in pyramidal neurons in the cerebral cortex of adult Kcnip2-EGFP/Rpl10a (NIDA115) mice.
Project description:TRAP translational profiling is a method that allows investigators to genetically characterize specific cell types in complex tissues such as mouse brain. Using this technique we obtained RNA-Seq data from actively translating transcripts present in pericytes in the cerebral cortex of adult Gdnf-EGFP/Rpl10a (ES2243) mice
Project description:TRAP translational profiling is a method that allows investigators to genetically characterize specific cell types in complex tissues such as mouse brain. Using this technique we obtained RNA-Seq data from actively translating transcripts present in interneurons in the cerebral cortex of adult Htr3a-Cre (NO176) mice x Rosa26-LSL-EGFP/Rpl10a mice
Project description:TRAP translational profiling is a method that allows investigators to genetically characterize specific cell types in complex tissues such as mouse brain. Using this technique we obtained RNA-Seq data from actively translating transcripts present in pyramidal neurons in the cerebral cortex of adult S100a10-EGFP/Rpl10a (ES691) mice that were administered either saline or cocaine.
Project description:TRAP translational profiling is a method that allows investigators to genetically characterize specific cell types in complex tissues such as mouse brain. Using this technique we obtained RNA-Seq data from actively translating transcripts present in pyramidal neurons in the cerebral cortex of adult Glt25d2-EGFP/Rpl10a (DU9) mice that were administered either saline or cocaine.
Project description:TRAP translational profiling is a method that allows investigators to genetically characterize specific cell types in complex tissues such as mouse brain. Using this technique we obtained RNA-Seq data from actively translating transcripts present in neurons in the cerebral cortex and dentate gyrus of adult Rbp4-Cre (KL100) mice x Rosa26-LSL-EGFP/Rpl10a mice.
Project description:In mammalian genomes a subset of genes is regulated by genomic imprinting resulting in silencing of one parental allele. Imprinting is essential for cerebral cortex development but prevalence and functional impact in individual cells is unclear. Here we determined allelic expression in cortical cell types and established a quantitative platform to interrogate imprinting in single cells. We created cells with uniparental disomy (UPD) containing either two copies of the maternal or paternal chromosome hence imprinted genes will be twofold overexpressed or not expressed. By genetic labeling of UPD we determined cellular phenotypes and transcriptional responses to deregulated imprinted gene expression at unprecedented single cell resolution. We discovered an unexpected degree of cell type specificity and a novel function of imprinting in the regulation of cortical astrocyte survival. More generally our results suggest functional relevance of imprinted gene expression in glial astrocyte lineage and thus for generating cortical cell type diversity.
