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Functional analysis of CFA/I ETEC H10407 strain (1)

ABSTRACT: Functional analysis of ETEC adaptation to survival in water and in vivo colonisation

PROVIDER: PRJEB38958 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR4330710.fastq.gz	Fastqsanger.gz
	ERR4330711.fastq.gz	Fastqsanger.gz
	ERR4330712.fastq.gz	Fastqsanger.gz
	ERR4330703.fastq.gz	Fastqsanger.gz
	ERR4330713.fastq.gz	Fastqsanger.gz

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Global transcriptome of CFA/I expressing ETEC H10407 under in vitro growth and water stress

Project description:Enterotoxigeneic Escherichia coli (ETEC) is a leading cause of diarrhoeal infections in young children living in endemic regions in low and middle-income countries and adults travelling to these destinations. CFA/I fimbriae have been identified as the predominant colonisation factor associated with human ETEC infections. Here we used used Transposon-directed insertion-site sequencing (TraDIS) and transcriptomic analysis to identify the essential genome of the prototypical CFA/I expressing ETEC strain H10407 and uncover the survival mechanisms that enhance persistence of ETEC isolates in water and within mammalian hosts. RNA transcription profiles of H10407 were identified under different in vitro growth conditions including aerobic growth in neutral LB media (pH7); aerobic growth in acidic media (pH5); aerobic growth in alkaline media (pH9); anaerobic growth in neutral LB media (pH7); and survival in fresh water. Research work including RNA preparation and bioinformatics and statistical analyses were conducted at the Wellcome Trust Sanger Institute (WTSI) and the Westmead Institute for Medical Research (WIMR), the University of Sydney. The sequencing data was generated in the Bioscience Core Laboratory at King Abdullah University of Science and Technology (KAUST).

2021-04-21 | E-MTAB-9364 | biostudies-arrayexpress

ETEC H10407 ChIP-Seq

Project description:ETEC H10407 ChIP-Seq

| PRJEB7505 | ENA

Use of an ETEC proteome microarray to evaluate cross-reactivity of ETVAX® vaccine-induced IgG antibodies in Zambian children

Project description:Enterotoxigenic E. coli (ETEC) is a major cause of moderate to severe diarrhoea in low-middle income countries (LMICs) and affects mostly children and travelers to endemic regions. ETEC are highly diverse pathovar with over 25 colonisation factors (fimbriae) described. The development of a broadly protective vaccine to cover most of the ETEC variants is critical for disease control. This study evaluated whether vaccination with ETVAX®; the most clinically advanced ETEC vaccine candidate results in IgG responses that cross-react with other ETEC antigens/ colonisation factors not overexpressed in the vaccine. A proteome microarray was used to assess IgG responses before and after vaccination to gain insight in the antigenic composition of ETEC that children in Zambia are exposed to and to see whether ETVAX® provides a level of cross-protection against antigens not expressed in the vaccine. The study shows that ETVAX® containing CFA/1, elicits antibodies that cross-react with other class 5 fimbriae and has the potential to offer broad protection. We also see that other antigens apart from classical antigens are immunodominant and possibly play a role in ETEC pathogenesis and may need to be investigated for their role in protection

2023-05-31 | GSE220814 | GEO

Escherichia coli ETEC H10407

Project description:Escherichia coli ETEC H10407 genome sequencing project

| PRJEA42749 | ENA

Escherichia coli ETEC H10407

Project description:transcriptome of ETEC from various clincial presentations

| PRJNA171249 | ENA

Escherichia coli ETEC H10407

Project description:transcriptome of ETEC from various clincial presentations

| PRJNA171248 | ENA

Expression data of enterotoxigenic (ETEC) isolate E24377A

2012-03-01 | GSE31804 | GEO

Expression data of enterotoxigenic (ETEC) isolate E24377A

Project description:ETEC is an important human pathogen. Although the mechanism of diarrhea is known in ETEC, the regulatory networks are less understood. This study was conducted to understand the global expression of ETEC isolate E24377A under different growth and environemental conditions. ETEC isolate E24377A was grown in the presence of several chemical signals, including bile salts, glucose, and pre-conditioned media (PCM) from other enteric pathogens. E24377A was also grown to different densities, to see if a quorum sensing mechanism was in place The isolate was grown in different types of media, with different ammendments, and at different growth densities. The overall goal was to determine how expression gene expression changes in the presence of chemical signals; a special emphasis was placed on the expression of known and suspected virulence and colonization factors

2012-03-01 | E-GEOD-31804 | biostudies-arrayexpress

MeDIP-chip for porcine intestinal epithelial cells with ETEC F4ab/ac infection

Project description:To better understand the molecular effects of Enterotoxigenic Escherichia coli (ETEC) F4ab/ac infection, we performed a genome-wide comparison of the changes in DNA methylation in ETEC F4ab/ac infected porcine intestinal epithelial cells. Our data provides further insight into the epigenetic alterations of ETEC F4ab/ac infected porcine intestinal epithelial cells and may advance the identification of biomarkers and drug targets for predicting susceptibility to and controlling ETEC F4ab/ac induced diarrhea.

2020-04-06 | GSE143661 | GEO

ETEC H10407 ChIP-Seq

Project description:To generate and compile data from ChIP-Seq libraries. Looking at CRP, H-NS, and sigma70 binding genome wide under M9 minimal growth conditions in duplicate.

2014-10-16 | E-MTAB-2917 | biostudies-arrayexpress

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