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RNA-seq of MG1655 wt and MG1655 delta-phoB strains grown in MOPS media with low phosphate (0.2 mM)

ABSTRACT: RNA-seq of MG1655 wt and MG1655 delta-phoB strains grown in MOPS media with low phosphate (0.2 mM)

PROVIDER: PRJEB39184 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR4314141.fastq.gz	Fastqsanger.gz
	ERR4314142.fastq.gz	Fastqsanger.gz
	ERR4314143.fastq.gz	Fastqsanger.gz
	ERR4314144.fastq.gz	Fastqsanger.gz

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ChIP-seq of MG1655 wt, MG1655 ΔphoB, MG1655 Δhns, MG1655 ΔphoB+Δhns and MG1655 PhoB-FLAG strains grown in MOPS media with either low phosphate (0.2 mM) or high phosphate (1.32 mM)

Project description:We used ChIP-seq to map the binding of of C-terminally FLAG3-tagged PhoB to the Escherichia coli K-12 genome during growth in MOPS minimal media with low phosphate or high phosphate (0.2 mM or 1.32 mM, respectively). As a control, we performed ChIP-seq in an untagged strain. We also used ChIP-seq to map Sigma 70 (associates with initiating RNA polymerase) binding across the E. coli K-12 genome in wild-type, ΔphoB, Δhns, and Δhns ΔphoB strains grown in low phosphate medium to determine whether PhoB modulates recruitment of RNA polymerase to promoters and whether this is modulated by H-NS.

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RNA-seq of MG1655 wt and MG1655 delta-phoB strains grown in MOPS media with low phosphate (0.2 mM)

Project description:RNA-seq of MG1655 wt and MG1655 delta-phoB strains grown in MOPS media with low phosphate (0.2 mM)

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RNA-seq of MG1655 wt and MG1655 delta-phoB strains grown in MOPS media with low phosphate (0.2 mM)

Project description:We used RNA-seq to determine differences in RNA levels between wild-type E. coli K-12 and a ΔphoB mutant, each grown in MOPS media with low phosphate (0.2 mM).

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Project description:We used RNA-seq to determine differences in RNA levels between wild-type E. coli K-12 and a ΔphoB mutant, each grown in MOPS media with low phosphate (0.2 mM).

2020-10-03 | E-MTAB-9591 | biostudies-arrayexpress

ChIP-seq of MG1655 wt, MG1655 ΔphoB, MG1655 Δhns, MG1655 ΔphoB+Δhns and MG1655 PhoB-FLAG strains grown in MOPS media with either low phosphate (0.2 mM) or high phosphate (1.32 mM)

Project description:ChIP-seq of MG1655 wt, MG1655 ΔphoB, MG1655 Δhns, MG1655 ΔphoB+Δhns and MG1655 PhoB-FLAG strains grown in MOPS media with either low phosphate (0.2 mM) or high phosphate (1.32 mM)

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Ribosome profiling of E. coli K-12 MG1655 MOPS rich media with 0.2% glucose

Project description:Ribosome profiling was performed on E. coli wild type cells. All replicates were grown to an OD of ~0.4 in MOPS rich Media with 0.2% glucose supplemented, aerobically in shake flasks. Cultures were treated with chloramphenicol 2 min prior to harvest.

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Ribosome profiling of E. coli K-12 MG1655 MOPS rich media with 0.2% glucose

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Escherichia coli O157:H7 responds to phosphate starvation by modifying LPS involved in biofilm formation

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